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ABS1008

Sigma-Aldrich

Anti-phospho Glucocorticoid Receptor (Ser134) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):
Glucocorticoid receptor, Ser134-phosphorylated, GR, Ser134-phosphorylated, Nuclear receptor subfamily 3 group C member 1, Ser134-phosphorylated
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, rat

species reactivity (predicted by homology)

sheep (based on 100% sequence homology), canine (based on 100% sequence homology), horse (based on 100% sequence homology), feline (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... NR3C1(2908)

General description

Glucocorticoid receptor (UniProt P04150; also known as GR, Nuclear receptor subfamily 3 group C member 1) is encoded by the NR3C1 (also known as GCCR, GCR, GCRST, GRL) gene (Gene ID 2908) in human. Glucocorticoid receptor is widely expressed in almost all cell types and regulates genes that control development, metabolism, and immune response upon binding glucocorticoids. Hormone-bound GR translocates from the cytoplasm to the nucleus where it regulates gene transcriptions by binding to glucocorticoid response elements (GREs) or by interacting with various transcription factors. Alternatively, hormone-bound GR can represses gene transcriptions by blocking the nuclear translocation of transcription factors. BDNF-stimulated TrkB activation is shown to induce GR phosphorylation at Ser155 and Ser287 in primary rat cortical neurons (equivalent to human GR Ser134 and Ser267), resulting in enhanced GR transcription activity. GR is composed of an N-terminal modulating domain (a.a. 1-420), a DNA-binding domain (a.a. 421-486), and a C-terminal ligand-binding domain (a.a. 528-777).

Specificity

The target phosphorylation site recognized by this polyclonal antibody is present in all 9 human GR spliced isoforms (Ser108 of isoforms Alpha-B and Beta-B; Ser134 of all the other isoforms) and in both rat GR spliced isoforms (Ser155 of isoform A and Ser128 of isoform B) reported by UniProt (P04150 and P06536, respectively).

Immunogen

Epitope: pSer134.
Linear peptide corresponding to a human glucocorticoid receptor sequence containing phosphorylated Ser134.

Application

Anti-phospho Glucocorticoid Receptor (Ser134) Antibody is an antibody against phospho Glucocorticoid Receptor for use in Western Blotting.
Research Category
Signaling
Research Sub Category
Hormones & Receptors
Western Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of endogenous GR in primary rat cortical neurons (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).
Western Blotting Analysis: A representative lot detected BDNF-induced Ser155 phosphorylation of exogenously expressed wild-type, but not S155A mutant, rat GR in transfected HEK293TrkB cells (Lambert, W.M., et al. (2013). Mol. Cell. Biol. 33(18):3700-3714).

Quality

Evaluated by Western Blotting in cell lysate from HEK293 expressing FLAG-tagged human glucocorticoid receptor.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Ser134 phosphorylation of FLAG-tagged human glucocorticoid receptor in 10 ug lysate from BDNF-treated HEK293 transfectants.

Target description

~85 kDa observed. 87.56 kDa (Rat isoforms A), 84.83 kDa (Rat isoforms B), 85.66 kDa (Human isoform Alpha; Alpha-A), 81.51 kDa (Human isoform Beta; Beta-A), 85.82 kDa (Human isoform Alpha-2; Gamma), 81.67 kDa (Human isoform Beta-2), 64.75 kDa (Human isoform GR-A alpha), 60.60 kDa (Human isoform GR-A beta), 82.85 kDa (Human isoform Alpha-B), 78.70 kDa (Human isoform Beta-B), 82.90 kDa (Human isoform 10; hGRDelta313-338) calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified
Purified rabbit polyclonal antibody in Tris-buffered saline (pH 8.0) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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