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ABS1068

Sigma-Aldrich

Anti-Acetyl CoA Carboxylase 1

from rabbit, purified by affinity chromatography

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Synonym(s):
ACC1, ACC-alpha, EC 6.4.1.2
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... ACACA(31)

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antibody form

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antibody form

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antibody form

affinity isolated antibody

clone

polyclonal

clone

10H2.1, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

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species reactivity

human

species reactivity

human, rat, mouse

species reactivity

rat, human, mouse

packaging

antibody small pack of 25 μL

packaging

antibody small pack of 25 μg

packaging

-

packaging

-

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

western blot: 1:500-1:2000

technique(s)

ELISA: 1:1000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

General description

Acetyl-CoA carboxylase 1 (UniProt: Q13085; also known as EC: 6.4.1.2, ACC1, ACC-alpha) is encoded by the ACACA (also known as ACAC, ACC1, ACCA) gene (Gene ID: 31) in human. ACC1 is a cytoplasmic protein that is expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues. It is also expressed at low level in pulmonary tissue, but is not detected in the liver. ACC1 can be present as a monomer, homodimer, or homotetramer. It can also form filamentous polymers. Four isoforms of ACC1 have been described that are produced by alternative splicing. It catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids and carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. ACC1 levels are regulated under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Mutations in ACACA gene can result in ACC1 deficiency and lead to error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.

Specificity

This rabbit polyclonal antibody detects Acetyl-CoA carboxylase 1 in human cells. It targets an epitope within 245 amino acids from the C-terminal region.

Immunogen

Epitope: C-terminus
GST-tagged recombinant fragment corresponding to 245 amino acids from the C-terminal half of human Acetyl-CoA carboxylase 1.

Application

Anti-Acetyl CoA Carboxylase 1, Cat. No. ABS1068, is a rabbit polyclonal antibody that detects Acetyl CoA Carboxylase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Acetyl CoA Carboxylase 1 in human liver and human kidney tissue sections.
Research Category
Signaling

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Acetyl CoA Carboxylase 1 in A431 cell lysate.

Target description

~220 kDa observed; 265.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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