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ABS1848

Sigma-Aldrich

Anti-Rho (-A, -B, -C) Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Transforming protein RhoA, h12, Rho-related GTP-binding protein RhoB, h6, Rho-related GTP-binding protein RhoC, h9
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

chicken (based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), mouse (based on 100% sequence homology), nematode (based on 100% sequence homology), Xenopus (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

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SAB4501661MABS1990MSAB1403218
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

7F7, monoclonal

clone

1E7, monoclonal

species reactivity

human

species reactivity

rat, mouse, human

species reactivity

rat, mouse, hamster, porcine, human, chicken, monkey, rabbit

species reactivity

human

technique(s)

western blot: suitable

technique(s)

ELISA: 1:1000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable

NCBI accession no.

NP_001655, NP_004031, NP_786886

NCBI accession no.

P61586

NCBI accession no.

NP_004031.1

NCBI accession no.

BC001338

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General description

Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2).

Specificity

This polyclonal antibody is raised against an internal sequence highly conserved among RhoA (100%), RhoB (95%), and RhoC (100%).

Immunogen

Epitope: Amino acids 73-94.
KLH-conjugated synthetic peptide (SYPDTDVILMCFSIDSPDSLEN-KK) corresponding to amino acids 73-94 of human RhoA & RhoB, with two C-terminal lysines added to increase peptide solubility corresponding to human Rho (-A, -B, -C).

Application

Anti-Rho (-A, -B, -C) Antibody is an antibody against Rho (-A, -B, -C) for use in Western Blotting.
Research Category
Signaling
Research Sub Category
G-proteins

Quality

Evaluated by Western Blotting in HL-60 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected RhoA family GTPase in 10 µg of HL-60 cell lysate.

Target description

~22 kDa observed. 21.44 kDa (human RhoA), 21.78 kDa (human RhoB), and 21.68 kDa (human RhoC) calculated.

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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