ABS530

Sigma-Aldrich

Anti-CDC7 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):
Cell division cycle 7-related protein kinase, CDC7-related kinase, HsCdc7, huCdc7
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology)

application(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

General description

CDC7, also known as CDC7-related kinase or HsCdc7/HuCdc7, and encoded by the gene CDC7/CDC7L1, is a critical kinase needed to phosphorylate critical substrates that regulate the G1/S phase transition and DNA replication. CDC7 is a serine-threonine kinase that forms a complex with either DBF4 or DBF4B proteins leading to the activation of the kinase activity necessary for its function. Additionally, CDC7 phosphorylates and forms a complex with MCM2 and MCM3 proteins which are highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. CDC7 is localized to the localized in the nucleus, and CDC7 is over expressed in many tumors and transformed cell lines.

Immunogen

KLH-conjugated linear peptide corresponding to the protein kinase domain of human CDC7.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Anti-CDC7 Antibody detects level of CDC7 & has been published & validated for use in CDC7.

Quality

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1 μg/mL of this antibody detected CDC7 in 10μg of human spleen tissue lysate.

Target description

~63 kDa observed

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity Purfied

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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