CDC7, also known as CDC7-related kinase or HsCdc7/HuCdc7, and encoded by the gene CDC7/CDC7L1, is a critical kinase needed to phosphorylate critical substrates that regulate the G1/S phase transition and DNA replication. CDC7 is a serine-threonine kinase that forms a complex with either DBF4 or DBF4B proteins leading to the activation of the kinase activity necessary for its function. Additionally, CDC7 phosphorylates and forms a complex with MCM2 and MCM3 proteins which are highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. CDC7 is localized to the localized in the nucleus, and CDC7 is over expressed in many tumors and transformed cell lines.
KLH-conjugated linear peptide corresponding to the protein kinase domain of human CDC7.
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Anti-CDC7 Antibody detects level of CDC7 & has been published & validated for use in CDC7.
Evaluated by Western Blotting in human spleen tissue lysate.
Western Blotting Analysis: 1 μg/mL of this antibody detected CDC7 in 10μg of human spleen tissue lysate.
~63 kDa observed
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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