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ABS79

Sigma-Aldrich

Anti-phospho-mTOR (Ser2159) Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapam
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), sheep (based on 100% sequence homology)

technique(s)

dot blot: suitable
inhibition assay: suitable (peptide)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer2159)

Gene Information

human ... MTOR(2475)

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SAB4501038SAB4301415MABS14
Anti-mTOR Antibody, clone F11 clone F11, from mouse

MABS14

Anti-mTOR Antibody, clone F11

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

species reactivity

human

species reactivity

rat, human, mouse

species reactivity

rat, human, mouse

species reactivity

human

UniProt accession no.

P42345

UniProt accession no.

P42345

UniProt accession no.

P42345

UniProt accession no.

P42346

technique(s)

dot blot: suitable, western blot: suitable, inhibition assay: suitable (peptide)

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

western blot: 1:500-1:1000 (Cell Lysate)

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

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General description

The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.

Specificity

This antibody recognizes mTOR phosphorylated at Ser2159.

Immunogen

Epitope: Phosphorylated at Ser2159
KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Ser2159.

Application

Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay, Dot Blot.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases
Western Blot Analysis: A representative lot detected phospho-mTOR (Ser2159) in HEK293 cell lysate. Image courtesy from the laboratory of Dr. Diane Fingar, University of Michigan Medical School.

Quality

Evaluated by Dot Blot in unmodified and modified mTOR peptides.

Dot Blot Specificity Analysis: Unmodified and modified mTOR peptides (see Table) were probed with Anti-phospho-mTOR (Ser2159) (1:1,000 dilution).

Target description

290 kDa calculated

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Unmodified and modified mTOR peptides

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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mTOR kinase domain phosphorylation promotes mTORC1 signaling, cell growth, and cell cycle progression.
Ekim, B; Magnuson, B; Acosta-Jaquez, HA; Keller, JA; Feener, EP; Fingar, DC
Molecular and cellular biology null
Lipotoxicity-induced STING1 activation stimulates MTORC1 and restricts hepatic lipophagy.
Liu, et al.
Autophagy, 18, 860-876 (2022)
Quan-Yu Zhang et al.
Hepatology (Baltimore, Md.), 66(3), 834-854 (2017-05-17)
Cellular repressor of E1A-stimulated genes (CREG), a novel cellular glycoprotein, has been identified as a suppressor of various cardiovascular diseases because of its capacity to reduce hyperplasia, maintain vascular homeostasis, and promote endothelial restoration. However, the effects and mechanism of
Kashif Aziz Khan et al.
PLoS pathogens, 17(1), e1009111-e1009111 (2021-01-08)
Antiviral innate immune response to RNA virus infection is supported by Pattern-Recognition Receptors (PRR) including RIG-I-Like Receptors (RLR), which lead to type I interferons (IFNs) and IFN-stimulated genes (ISG) production. Upon sensing of viral RNA, the E3 ubiquitin ligase TNF
Cagri Bodur et al.
The EMBO journal, 37(1), 19-38 (2017-11-19)
The innate immune kinase TBK1 initiates inflammatory responses to combat infectious pathogens by driving production of type I interferons. TBK1 also controls metabolic processes and promotes oncogene-induced cell proliferation and survival. Here, we demonstrate that TBK1 activates mTOR complex 1

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