ACK5010GS
Amicon® Pro Affinity Concentration Kit GST with 10kDa Amicon® Ultra-0.5 Device
Amicon Pro Affinity Concentration Kit GST - A Centrifugal Tool for purifying & concentrating GSH-tagged proteins.
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About This Item
material
Amicon Ultra styrene-butadiene device, Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding), polyethylene cap, polyethylene frit, polypropylene device (for exchange), polypropylene frit, polypropylene tube (AU0.5; for collection), polypropylene tube (for collection), polypropylene tube (holder)
sterility
non-sterile
product line
Amicon®
manufacturer/tradename
Amicon® Pro
parameter
10 mL sample volume
technique(s)
buffer exchange: suitable, protein purification: suitable (antibody labeling), protein purification: suitable (concentration), protein purification: suitable (enrichment)
L
12 cm (4.7 in.)
diam.
2.97 cm
filtration area
1 cm2
working volume
10 mL
pore size
10 kDa NMWCO
shipped in
wet ice
General description
Application
Features and Benefits
Physical form
Legal Information
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Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Storage Class
3 - Flammable liquids
Certificates of Analysis (COA)
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Related Content
bios-C-epdf-1043-1304-SP
Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.
E. coli transformation with poly-histidine tagged constructs represents a common vehicle for protein production. Initial screening of small-scale cultures is routinely performed to identify clones producing the highest amounts of protein with the desired form and function. As the demand for greater throughput at this level has increased, so has the need for process simplification and greater reproducibility. To meet this need, we have developed a condensed workflow that can be performed in a single device.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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