AP1160

Anti-NDRG1 Mouse mAb (2D7)

liquid, clone 2D7, Calbiochem^®

• Synonym(s): Anti-N-myc Downstream Regulator 1

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2D7, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... NDRG1(10397)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDRG1 protein.

Recognizes the ~40-45 kDa NDRG1 protein in HeLa cells and human endometrium tissue.

This Anti-NDRG1 Mouse mAb (2D7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of NDRG1.

Immunogen

Full-length, recombinant human NDRG1 (aa 1-395), expressed as a GST fusion protein

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (0.7 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human endometrium tissue, HeLa cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Jiang, K., et al. 2010. Scand. J. Gastroenterol45, 898.
Leth-Larsen R, et al. 2009. Mol. Cell Proteomics8, 1436.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable