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AP124A

Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, Alkaline Phosphatase conjugate

Chemicon®, from goat

eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

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This Item
AP136A12-448AP132A
biological source

goat

biological source

goat

biological source

goat

biological source

goat

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse

species reactivity

rat

species reactivity

rabbit

species reactivity

rabbit

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

General description

Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Mouse IgG (H+L)
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Specific for mouse IgG, heavy and light chain.

Application

EIA and Western blots: 1:5,000-1:50,000.

Immunohistochemistry: 1:1,000-1:2,000.

Optimal working dilutions must be determined by end user.
Goat anti-Mouse IgG Antibody, Alkaline Phosphatase conjugate is an antibody against Mouse IgG for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Linkage

Replaces: AP126A

Physical form

ImmunoAffinity Purified
Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with

15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

Storage and Stability

this product is stable for several weeks at 2–8°C as an undiluted liquid. DO NOT FREEZE. For

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Please enter the Product Number and Lot Number.

Certificate of Quality

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