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AP124A

Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, Alkaline Phosphatase conjugate

Chemicon®, from goat

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eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

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This Item
AP181AP181PAQ127
conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

unconjugated

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

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General description

Alkaline Phosphatase-conjugated Affinity Purified Goat anti-Mouse IgG (H+L)
Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Specific for mouse IgG, heavy and light chain.

Application

EIA and Western blots: 1:5,000-1:50,000.

Immunohistochemistry: 1:1,000-1:2,000.

Optimal working dilutions must be determined by end user.
Goat anti-Mouse IgG Antibody, Alkaline Phosphatase conjugate is an antibody against Mouse IgG for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Linkage

Replaces: AP126A

Physical form

ImmunoAffinity Purified
Lyophilized. Buffer = 0.01 M Tirs HCl, 0.25 M NaCl, pH 8.0 with

15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute with sterile distilled water to match the volume indicated on the vial label. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature.

Storage and Stability

this product is stable for several weeks at 2–8°C as an undiluted liquid. DO NOT FREEZE. For

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Inactivation of Chikungunya virus by 1,5 iodonapthyl azide.
Sharma, A; Gupta, P; Maheshwari, RK
Virology Journal null
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Shaw, et al.
Brain communications, 5, fcad067-fcad067 (2023)
Toshihiko Tsukamoto et al.
Cancer, 106(7), 1489-1497 (2006-03-07)
Lysosomal proteases are implicated in cancer progression and metastasis. In the current study, using subtraction cloning for genes that are differentially expressed in metastasis, the authors isolated a clone encoding ceroid lipofuscinosis, neuronal 2 (CLN2), which is a lysosomal serine
Samantha D Francis Stuart et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Choroid plexus (CP) sequesters cadmium and other metals, protecting the brain from these neurotoxins. These metals can induce cellular stress and modulate homeostatic functions of CP, such as solute transport. We previously showed in primary cultured neonatal rat CP epithelial
Szabolcs Takáts et al.
Autophagy, 11(8), 1209-1215 (2015-06-11)
The HOPS tethering complex facilitates autophagosome-lysosome fusion by binding to Syx17 (Syntaxin 17), the autophagosomal SNARE. Here we show that loss of the core HOPS complex subunit Vps16A enhances autophagosome formation and slows down Drosophila development. Mechanistically, Tor kinase is

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