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AP132P

Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, Peroxidase Conjugated

1 mg/mL (after reconstitution), Chemicon®

eCl@ss:
32160702
NACRES:
NA.46

Quality Level

biological source

goat

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

concentration

1 mg/mL (after reconstitution)

application(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

conjugate

peroxidase conjugate

shipped in

wet ice

General description

Affinity purified goat anti-rabbit, HRP conjugated.

Specificity

Specific for rabbit IgG, heavy and light chain.

Application

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.

Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.
This Goat anti-Rabbit IgG Antibody, Peroxidase Conjugated is validated for use in ELISA, IH, WB for the detection of Rabbit IgG.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Physical form

ImmunoAffinity Purified
Purified goat IgG conjugated to horseradish peroxidase in buffer containing 0.01M PBS,  pH 7.1, with 15mg/mL BSA. Lyophilized.
RECONSTITUTION:
Reconstitute with sterile distilled water to match the volume indicated on the vial label.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months from date of shipment.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Porcine CPEB1 is involved in Cyclin B translation and meiotic resumption in porcine oocytes.
Nishimura Y, Kano K, Naito K
Animal Science Journal = Nihon Chikusan Gakkaiho null
Functions of FZR1 and CDC20, activators of the anaphase-promoting complex, during meiotic maturation of swine oocytes.
Yamamuro, T; Kano, K; Naito, K
Biology of reproduction null
Preimplantation-embryo-specific cell-cycle regulation is attributable to a low expression of retinoblastoma protein rather than its phosphorylation.
Asuka Egashira,Kiyoshi Kano,Kunihiko Naito
Journal of Reproduction and Development null
Wataru Fujii et al.
Biology of reproduction, 85(6), 1124-1132 (2011-07-23)
CDK-activating kinase (CAK) phosphorylates threonine 161 (T161) of CDC2, a catalytic subunit of maturation/M-phase promoting factor (MPF), and is essential for MPF activation in mitosis. CAK has been thought to consist of a catalytic subunit, a regulatory subunit and an...
Phospholipase D in the human ocular surface and in pterygium.
Louis Tong, Jing Li, Jaime Chew, Donald Tan, Roger Beuerman
Cornea null

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