Affinity purified goat anti-rabbit, HRP conjugated.
Specific for rabbit IgG, heavy and light chain.
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
This Goat anti-Rabbit IgG Antibody, Peroxidase Conjugated is validated for use in ELISA, IH, WB for the detection of Rabbit IgG.
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Purified goat IgG conjugated to horseradish peroxidase in buffer containing 0.01M PBS, pH 7.1, with 15mg/mL BSA. Lyophilized.
Reconstitute with sterile distilled water to match the volume indicated on the vial label.
Storage and Stability
Maintain lyophilized product at 2-8°C for up to 12 months from date of shipment.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.