AP156P

Goat Anti-Rabbit IgG Antibody, Fc, HRP conjugate

0.8 mg/mL, Chemicon^®

• Synonym(s): Goat anti-Rabbit IgG
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 100
• conjugate: peroxidase conjugate
• antibody form: F(ab′)₂ fragment of affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: rabbit
• manufacturer/tradename: Chemicon^®
• concentration: 0.8 mg/mL
• technique(s): ELISA: suitable; western blot: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

Application

ELISA and Western Blotting 1:5,000 - 1:100,000

Immunohistochemistry 1:500 - 1:5,000

Optimal working dilutions must be determined by the end user.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

This Goat anti-Rabbit IgG Antibody, Fc, HRP conjugate is validated for use in ELISA, WB for the detection of Rabbit IgG.

Biochem/physiol Actions

Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rabbit IgG but not with the light chains on most rabbit immunoglobulins. No antibody was detected against rabbit IgM, or against immunoglobulin serum proteins, but antibodies may cross-react with immunoglobulins from other species.

Physical form

Liquid. Buffer =0.01M Sodium Phosphate, 0.25 M NaCl, pH 7.6, and mixed with 15mg/mL BSA as stabilizer containing no preservatives.

RECONSTITUTION:

Reconstitute in 2mL deionized water.

Preparation Note

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. Use sterile technique as formulation has no preservatives. For extended storage after reconstitution, we suggest adding an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C, with or without aliquoting for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3