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AP187C

Sigma-Aldrich

Goat Anti-Rabbit IgG Antibody, Cy3 conjugate, Species Adsorbed

1.5 mg/mL, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

CY3 conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rabbit

manufacturer/tradename

Chemicon®

concentration

1.5 mg/mL

technique(s)

immunofluorescence: suitable

shipped in

wet ice

target post-translational modification

unmodified

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This Item
AP183CAP181CAP106C
conjugate

CY3 conjugate

conjugate

CY3 conjugate

conjugate

CY3 conjugate

conjugate

CY3 conjugate

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

F(ab′)2 fragment of affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rabbit

species reactivity

rat

species reactivity

mouse

species reactivity

goat

concentration

1.5 mg/mL

concentration

1.4 mg/mL

concentration

1.3 mg/mL

concentration

-

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Specificity

Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)

FLUOROPHORE/ PROTEIN ABSORBANCE RATIO

Approximately 2.25-2.8(A552/A280) = 2.7

Application

Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Suggested dilution for most applications: 1:100-1:800 for most applications.

Optimal working dilutions must be determined by the end user.
This Goat anti-Rabbit IgG Antibody, Cy3 conjugate, Species Adsorbed is validated for use in IF for the detection of Rabbit IgG.

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05 Sodium Azide.

RECONSTITUTION:

Reconstitute to 1.5 mg/mL with sterile distilled water.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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