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AP308P

Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, (H+L) HRP conjugate

1 mg/mL, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

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This Item
AP106PAP136PAP309P
conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

species reactivity

mouse

species reactivity

goat

species reactivity

rat

species reactivity

human

isotype

IgG

isotype

IgG

isotype

IgG

isotype

IgG

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity purified immunoglobulin

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

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General description

The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer.

Specificity

Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.

Application

ELISA:
1:3,000 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

Immunohistochemistry/immunocytochemistry:
1:200-1:500 dilution can be used (Bullock, G.R., 1982; Javois, L.C., 1994).

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Mouse IgG Antibody, (H+L) HRP conjugate is validated for use in ELISA, IC, IH, WB for the detection of Mouse IgG.

Quality

Western Blot, chromogenic:
1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

Western Blot, chemiluminescent:
1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).

Physical form

Affinity purified
Purified Goat IgG conjugated to horseradish peroxidase in buffer containing liquid in 0.01M PBS, pH 7.1 with 15mg/mL BSA and 0.1% Kathon.

Storage and Stability

The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format. DO NOT FREEZE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation markEnvironment

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Antigen expression kinetics and immune responses of mice immunized with noninfectious simian-human immunodeficiency virus DNA.
Hegde, R; Liu, Z; Mackay, G; Smith, M; Chebloune, Y; Narayan, O; Singh, DK
Journal of virology null
Kang Wang et al.
Nature communications, 11(1), 4419-4419 (2020-09-06)
Echovirus 30 (E30), a serotype of Enterovirus B (EV-B), recently emerged as a major causative agent of aseptic meningitis worldwide. E30 is particularly devastating in the neonatal population and currently no vaccine or antiviral therapy is available. Here we characterize
Jingsong Cao et al.
Experimental and therapeutic medicine, 19(1), 741-747 (2020-01-04)
In ABO-incompatible (ABOi) kidney transplantation (KT), antibodies can mediate immunological accommodation or immune rejection, but the mechanism by which B cells are induced to produce antibodies with different functions is still unclear. Previous research established an ABOi kidney cell model
Hong Liu et al.
Oncology reports, 42(6), 2759-2767 (2019-10-23)
MicroRNAs (miRs) are a class of non‑coding small RNAs that have been demonstrated to be involved in the pathogenesis of human cancer. There is even evidence that microRNAs can act as oncogenes or tumor suppressors. Although microRNA expression profiles have
Gali Epstein Shochet et al.
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Idiopathic pulmonary fibrosis (IPF) is a chronic and ultimately fatal disease characterized by a progressive decline in lung function. Fibrotic diseases, such as IPF, are characterized by uncontrolled activation of fibroblasts. Since the microenvironment is known to affect cell behavior

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