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APOMAG-62K

Millipore

MILLIPLEX® Human Apolipoprotein Magnetic Bead Panel - Cardiovascular Disease Multiplex Assay

This Bead-Based Multiplex Assay, using the Luminex® xMAP® technology, enables the simultaneous analysis of Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E.

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Synonym(s):
human apolipoprotein CVD immunoassay panel, luminex human apolipoprotein cardiovascular disease multiplex assay, millipore human apolipoprotein CVD multiplex kit
eCl@ss:
32161000

feature

This Bead-Based Multiplex Assay, using the Luminex® xMAP® technology, enables the simultaneous analysis of Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E.

Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 85%
(Apo E)
accuracy: 86%
(Apo AI)
accuracy: 89%
(Apo CIII)
accuracy: 92%
(Apo B)
sensitivity: 1.00 ng/mL
(Apo AII)
sensitivity: 1.22 ng/mL
(Apo CII)
sensitivity: 1.42 ng/mL
(Apo E)
sensitivity: 1.48 ng/mL
(Apo AI)
sensitivity: 1.71 ng/mL
(Apo CIII)
sensitivity: 4.84 ng/mL
(Apo B)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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MILLIPLEX® Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay The Human Metabolic Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 13 protein biomarkers in human serum, plasma and cell/tissue culture samples.

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manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 85%
(Apo E), accuracy: 86%
(Apo AI), accuracy: 89%
(Apo CIII), accuracy: 92%
(Apo B), sensitivity: 1.00 ng/mL
(Apo AII), sensitivity: 1.22 ng/mL
(Apo CII), sensitivity: 1.42 ng/mL
(Apo E), sensitivity: 1.48 ng/mL
(Apo AI), sensitivity: 1.71 ng/mL
(Apo CIII), sensitivity: 4.84 ng/mL
(Apo B)

assay range

accuracy: 102%
(Haptoglobin), accuracy: 109%
(AGP), accuracy: 113%
(Platelet Factor-4), accuracy: 117%
(Adipsin), standard curve range: 0.004-15 ng/mL
(L-Selectin & SAP), standard curve range: 0.012-50 ng/mL
(CRP), standard curve range: 0.037-150 ng/mL
(PF4), standard curve range: 0.061-250 ng/mL
(HPTGN), standard curve range: 0.098-400 ng/mL
(AGP & Adipsin), standard curve range: 0.244-1,000 ng/mL
(Fetuin A & vWF), standard curve range: 0.488-2,000 ng/mL
(A2M)

assay range

accuracy: 103%
(Thrombomodulin), accuracy: 104%
(Pentraxin-3 (PTX3)), accuracy: 104%
(Tissue Factor (TF)), accuracy: 106%
(Pecam-1), accuracy: 109%
(sE-Selectin), standard curve range: 0.01-5 ng/mL
(Troponin T (TnT)), standard curve range: 0.04-30 ng/mL
(Follistatin (FST)), standard curve range: 0.14-100 ng/mL
(Pentraxin-3 (PTX3) & Tissue Factor (TF)), standard curve range: 0.27-200 ng/mL
(Pecam-1), standard curve range: 0.34-250 ng/mL
(Thrombomodulin), standard curve range: 2.7-2,000 ng/mL
(sE-Selectin), inter-assay cv: <20%
intra-assay cv: <10%

assay range

accuracy: 101%
(Glucagon), accuracy: 102%
(Leptin), accuracy: 103%
(GIP), accuracy: 103%
(GLP-1 (Total)), accuracy: 105%
(Ghrelin (Active)), accuracy: 106%
(MCP-1), accuracy: 97%
(TNFα), accuracy: 99%
(C-Peptide), sensitivity: 0.5 pg/mL
(MinDC+2SD; TNF?), sensitivity: 1.1 pg/mL
(MinDC+2SD; GIP), sensitivity: 13 pg/mL
(MinDC+2SD; IL-6), sensitivity: 14 pg/mL
(MinDC+2SD; Ghrelin (Active)), sensitivity: 14 pg/mL
(MinDC+2SD; MCP-1), sensitivity: 146 pg/mL
(MinDC+2SD; PYY), sensitivity: 15 pg/mL
(MinDC+2SD; Amylin (Total)), sensitivity: 16 pg/mL
(MinDC+2SD; C-Peptide), sensitivity: 16 pg/mL
(MinDC+2SD; Glucagon), sensitivity: 160 pg/mL
(MinDC+2SD; Insulin), sensitivity: 2.4 pg/mL
(MinDC+2SD; GLP-1 (Active)), sensitivity: 2.9 pg/mL
(MinDC+2SD; PP), sensitivity: 28 pg/mL
(MinDC+2SD; Amylin (Active)), sensitivity: 64 pg/mL
(MinDC+2SD; Leptin), sensitivity: 7.3 pg/mL
(MinDC+2SD; GLP-1 (Total)), standard curve range: 0.69-500 pg/mL
(TNFα), standard curve range: 1.4-1,000 pg/mL
(GIP), standard curve range: 13.7-10,000 pg/mL
(Amylin (Active)), standard curve range: 13.7-10,000 pg/mL
(Amylin (Total)), standard curve range: 13.7-10,000 pg/mL
(Ghrelin (Active)), standard curve range: 13.7-10,000 pg/mL
(Glucagon), standard curve range: 13.7-10,000 pg/mL
(IL-6), standard curve range: 13.7-10,000 pg/mL
(MCP-1), standard curve range: 13.7-10,000 pg/mL
(PYY), standard curve range: 137-100,000 pg/mL
(Insulin), standard curve range: 137-100,000 pg/mL
(Leptin), standard curve range: 2.7-2,000 pg/mL
(GLP-1 (Active)), standard curve range: 2.7-2,000 pg/mL
(GLP-1 (Total)), standard curve range: 2.7-2,000 pg/mL
(PP), standard curve range: 27.4-20,000 pg/mL
(C-Peptide), inter-assay cv: <15%
intra-assay cv: <10%
(C-Peptide), inter-assay cv: <15%
intra-assay cv: <10%
(GIP), inter-assay cv: <15%
intra-assay cv: <10%
(GLP-1 (Active)), inter-assay cv: <15%
intra-assay cv: <10%
(GLP-1 (Total)), inter-assay cv: <15%
intra-assay cv: <10%
(Ghrelin (Active)), inter-assay cv: <15%
intra-assay cv: <10%
(Glucagon), inter-assay cv: <15%
intra-assay cv: <10%
(IL-6), inter-assay cv: <15%
intra-assay cv: <10%
(Insulin), inter-assay cv: <15%
intra-assay cv: <10%
(Leptin), inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1), inter-assay cv: <15%
intra-assay cv: <10%
(PP), inter-assay cv: <15%
intra-assay cv: <10%
(PYY), inter-assay cv: <15%
intra-assay cv: <10%
(TNFα), inter-assay cv: <20%
intra-assay cv: <10%
(Amylin (Active)), inter-assay cv: <20%
intra-assay cv: <10%
(Amylin (Total))

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Metabolic Syndrome has received increased attention from the medical community with increasing cases of obesity, type 2 diabetes and related cardiovascular diseases being described and studied.

MILLIPLEX® Human Apolipoprotein Panel is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue culture supernatant or other biological samples: APOA-1, APOA-2, APOB, APOC-2, APOC-3, and APOE. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular

Specificity

Cross Reactivty
To test cross-reactivity among the assays in the panel, individual apolipoprotein standard was prepared at a concentration 4 times greater than the highest concentration in the calibration curve and tested in the 6-plex assays with multiplexed beads containing immobilized capture antibodies and individual detection antibodies. There was negligible cross-reactivity within the panel.

Application

  • Analytes: Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E
  • Recommended Sample Type: Human serum, plasma, tissue culture supernatant or other biological samples
  • Recommended Sample Dilution: 10 μL of a 1: 4,000 dilution of plasma or serum
  • Assay Run Time: Primary incubation of 1 hour followed by a secondary incubation of 30 minutes, both at room temperature (20-25°C)
  • Research Subcategory: Cardiovascular Disease

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.
Standard curve range: Refer to the kit protocol for details.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

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Anastasiia S Boiko et al.
Heliyon, 5(7), e02033-e02033 (2019-07-19)
Schizophrenia is associated with a lowered life expectancy due to cardiovascular disease. This is, at least in part, related to an increased vulnerability to the development of metabolic syndrome (MetS) in patients with schizophrenia. The dysregulation of apolipoproteins (Apos) may
Anna Posod et al.
Journal of the American Heart Association, 8(8), e011199-e011199 (2019-04-11)
Background Little is known about plasma apolipoprotein profiles in very preterm-born and term-born preschool children compared with the adult population. This is of particular interest because apolipoprotein composition might contribute to cardiometabolic outcome in later life. Methods and Results Children
Consumption of buckwheat modulates the post-prandial response of selected gastrointestinal satiety hormones in individuals with type 2 diabetes mellitus.
Stringer, DM; Taylor, CG; Appah, P; Blewett, H; Zahradka, P
Metabolism, Clinical and Experimental null
Xiaoke Yin et al.
Circulation. Cardiovascular genetics, 10(6) (2017-12-15)
Mass spectrometry is selective and sensitive, permitting routine quantification of multiple plasma proteins. However, commonly used nanoflow liquid chromatography (LC) approaches hamper sample throughput, reproducibility, and robustness. For this reason, most publications using plasma proteomics to date are small in
Yi-Ting Chen et al.
Molecular & cellular proteomics : MCP, 16(5), 799-811 (2017-02-27)
Multiple (selected) reaction monitoring (MRM/SRM) of peptides is a growing technology for target protein quantification because it is more robust, precise, accurate, high-throughput, and multiplex-capable than antibody-based techniques. The technique has been applied clinically to the large-scale quantification of multiple
View All Related Papers

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