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APOMAG-62K

MILLIPLEX® Human Apolipoprotein Panel

This Bead-Based Multiplex Assay, using the Luminex® xMAP® technology, enables the simultaneous analysis of Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E., Configurable Human Apolipoprotein 6-Plex Panel

Synonym(s):

human apolipoprotein CVD immunoassay panel, luminex human apolipoprotein cardiovascular disease multiplex assay, millipore human apolipoprotein CVD multiplex kit, Apo protein detection panel, apolipoprotein biomarker panel, cardiovascular lipid profile, human apolipoprotein multiplex kit, human cholesterol transport marker panel, lipid metabolism assay

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000

Key Documents

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Quality Level

200

feature

This Bead-Based Multiplex Assay, using the Luminex® xMAP® technology, enables the simultaneous analysis of Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E.

species reactivity

human

assay range

accuracy: 85%
(Apo E), accuracy: 86%
(Apo AI), accuracy: 89%
(Apo CIII), accuracy: 92%
(Apo B), sensitivity: 1.00 ng/mL
(Apo AII), sensitivity: 1.22 ng/mL
(Apo CII), sensitivity: 1.42 ng/mL
(Apo E), sensitivity: 1.48 ng/mL
(Apo AI), sensitivity: 1.71 ng/mL
(Apo CIII), sensitivity: 4.84 ng/mL
(Apo B)

technique(s)

multiplexing: suitable

input

serum plasma (Cell culture, cell culture supernatants)

packaging

pkg of 1 ea

manufacturer/tradename

Milliplex®

detection method

fluorometric (Luminex® xMAP® technology)

shipped in

wet ice

storage temp.

2-8°C

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MILLIPLEX® Human Apolipoprotein Panel This Bead-Based Multiplex Assay, using the Luminex® xMAP® technology, enables the simultaneous analysis of Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E., Configurable Human Apolipoprotein 6-Plex Panel

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MILLIPLEX® Mouse Adipokine Panel Configurable Mouse Adipokine 7-Plex Panel

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species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

mouse

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 85%
(Apo E), accuracy: 89%
(Apo CIII), sensitivity: 1.00 ng/mL
(Apo AII), sensitivity: 1.42 ng/mL
(Apo E), sensitivity: 1.71 ng/mL
(Apo CIII), accuracy: 86%
(Apo AI), sensitivity: 1.22 ng/mL
(Apo CII), sensitivity: 4.84 ng/mL
(Apo B), accuracy: 92%
(Apo B), sensitivity: 1.48 ng/mL
(Apo AI)

assay range

accuracy: 102%
(Haptoglobin), accuracy: 109%
(AGP), accuracy: 113%
(Platelet Factor-4), accuracy: 117%
(Adipsin), standard curve range: 0.004-15 ng/mL
(L-Selectin & SAP), standard curve range: 0.012-50 ng/mL
(CRP), standard curve range: 0.037-150 ng/mL
(PF4), standard curve range: 0.061-250 ng/mL
(HPTGN), standard curve range: 0.098-400 ng/mL
(AGP & Adipsin), standard curve range: 0.244-1,000 ng/mL
(Fetuin A & vWF), standard curve range: 0.488-2,000 ng/mL
(A2M)

assay range

accuracy: 103%
(Thrombomodulin), accuracy: 104%
(Pentraxin-3 (PTX3)), accuracy: 104%
(Tissue Factor (TF)), accuracy: 106%
(Pecam-1), accuracy: 109%
(sE-Selectin), standard curve range: 0.01-5 ng/mL
(Troponin T (TnT)), standard curve range: 0.04-30 ng/mL
(Follistatin (FST)), standard curve range: 0.14-100 ng/mL
(Pentraxin-3 (PTX3) & Tissue Factor (TF)), standard curve range: 0.27-200 ng/mL
(Pecam-1), standard curve range: 0.34-250 ng/mL
(Thrombomodulin), standard curve range: 2.7-2,000 ng/mL
(sE-Selectin), inter-assay cv: <20%
intra-assay cv: <10%

assay range

accuracy: 77-92%, standard curve range: 12.2-50,000 pg/mL

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Apolipoproteins are essential components of lipoproteins and play critical roles in lipid transport, metabolism, and cardiovascular health. This panel—Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, and Apo E—captures key indicators of cholesterol regulation, lipoprotein structure, and atherogenesis. These analytes are valuable for studying dyslipidemia, metabolic syndrome, and cardiovascular disease, supporting both translational and clinical lipid research

Application

MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 6 analytes human serum, plasma, or cell culture supernatants.

Analytes Included: Apo AI, Apo AII, Apo B, Apo CII, Apo CIII, Apo E




Assay Characteristics: Refer to assay protocol for details on cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

Features:
  • Targets key apolipoproteins involved in lipid transport and metabolism
  • Includes 6 analytes relevant to cardiovascular health and dyslipidemia
  • Multiplex format enables simultaneous detection from minimal sample volume
  • Compatible with human serum and plasma samples
  • Designed for research in lipid disorders, atherosclerosis, and metabolic syndrome
Benefits:
  • Provides a comprehensive profile of apolipoproteins for cardiovascular risk assessment
  • Supports investigation of lipid metabolism and transport mechanisms
  • Facilitates evaluation of therapeutic interventions targeting dyslipidemia
  • Enables high-throughput analysis with reduced sample and reagent use

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

target_organs

Respiratory Tract

Storage Class

10 - Combustible liquids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiaoke Yin et al.
Circulation. Cardiovascular genetics, 10(6) (2017-12-15)
Mass spectrometry is selective and sensitive, permitting routine quantification of multiple plasma proteins. However, commonly used nanoflow liquid chromatography (LC) approaches hamper sample throughput, reproducibility, and robustness. For this reason, most publications using plasma proteomics to date are small in
Consumption of buckwheat modulates the post-prandial response of selected gastrointestinal satiety hormones in individuals with type 2 diabetes mellitus.
Stringer, DM; Taylor, CG; Appah, P; Blewett, H; Zahradka, P
Metabolism, Clinical and Experimental null
Yi-Ting Chen et al.
Molecular & cellular proteomics : MCP, 16(5), 799-811 (2017-02-27)
Multiple (selected) reaction monitoring (MRM/SRM) of peptides is a growing technology for target protein quantification because it is more robust, precise, accurate, high-throughput, and multiplex-capable than antibody-based techniques. The technique has been applied clinically to the large-scale quantification of multiple
Elise Grytten et al.
Clinical nutrition (Edinburgh, Scotland), 40(5), 2556-2575 (2021-05-03)
Marine-derived omega-3 (n-3) polyunsaturated fatty acids (PUFAs), mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), lower circulating levels of triacylglycerols (TAGs), and the plant-derived omega-6 (n-6) PUFA linoleic acid (LA) may reduce cholesterol levels. Clinical studies on effects of these
Anastasiia S Boiko et al.
Heliyon, 5(7), e02033-e02033 (2019-07-19)
Schizophrenia is associated with a lowered life expectancy due to cardiovascular disease. This is, at least in part, related to an increased vulnerability to the development of metabolic syndrome (MetS) in patients with schizophrenia. The dysregulation of apolipoproteins (Apos) may
View All Related Papers

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Protocol: Human Apolipoprotein Magnetic Bead Panel
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Questions

  1. Can you provide information on whether Milliplex Human Apolipoprotein Magnetic Bead Panel kit measures a specific type of apolipoprotein proteoform or if it includes measurements for all types of proteoforms? (Cat# APOMAG-62K)

    1 answer

    1. The Milliplex Human Apolipoprotein Magnetic Bead Panel includes the following components:

      - APO AI: It consists of a capture antibody (monoclonal) designed against purified full length human apolipoprotein A1, and a detection antibody (monoclonal) specific to human APO A I, whether purified or in the form of HDL.

      - APO AII: This includes a capture antibody (polyclonal) specific for human apolipoprotein A II, and a detection antibody (polyclonal) also specific to human APO II.

      - APO B: It comprises a capture antibody (monoclonal) specific for native human APO B in the form of LDL, and a detection antibody (monoclonal) specific to human APO B in the form of LDL.

      - APO C II: This involves a capture antibody (polyclonal) specific to human APO CII, with a corresponding detection antibody (polyclonal) specific to human APO C II.

      - APO C III: It contains a capture antibody (polyclonal) specific to human APO CIII, along with a detection antibody (polyclonal) specific to human APO C III.

      - APO E: This component includes a capture antibody (polyclonal) specific to human APO E, and a detection antibody (polyclonal) specific to human APO E.

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