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BI0166

Sigma-Aldrich

Activator Reagent 0.3M BMT in AcN

Biosynthesis Novabiochem®

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Quality Level

product line

Novabiochem®

form

liquid

reaction suitability

reaction type: Coupling Reactions
reagent type: catalyst

transition temp

flash point 2 °C (Acetonitrile)

shipped in

ambient

storage temp.

2-30°C

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This Item
BI015826001KL033000
vibrant-m

BI0166

Activator Reagent 0.3M BMT in AcN

vibrant-m

26001K

MAPTrix Reagent

vibrant-m

L033000

BTT Activator

Quality Level

200

Quality Level

200, 300

Quality Level

-

Quality Level

200

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-8°C

storage temp.

-

form

liquid

form

liquid

form

lyophilized powder

form

-

product line

Novabiochem®

product line

Novabiochem®

product line

-

product line

Proligo Reagents

transition temp

flash point 2 °C (Acetonitrile)

transition temp

flash point 5.6 °C

transition temp

-

transition temp

-

General description

Activator Reagent 0.3M 5-benzylmercaptotetrazole (BMT) in acetonitrile (AcN) is also known as benzylthiotetrazole (BTT) or benzyl-1H-tetrazole). It is used as a phosphoramidite activator reagent for oligonucleotide synthesis.

Application

0.3M BMT in AcN can be used as a reagent in solid phase phosphorylation of the oligomers. The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the 5′hydroxyl group of the growing oligonucleotide chain. The high activation potential of this reagent may be due to its greater acidity (pKa=4.08), which makes it easier to protonate the trivalent phosphorus in the initial stage of activation and increase the speed of the coupling reaction.

Features and Benefits

  • efficient and rapid activation reagent that produces less
  • phosphoramidite waste      
  • higher solubility at lower temperatures      
  • does not crystallize or clog lines       
  • safer reagent to use

Analysis Note

Color (APHA): 10APHA max
Component: Acetonitrile Purity (By GC): 99.9% min
Form: Clear Liquid
Identity (IR-spectrum): Conforms
Molarity of 5-Benzylmercaptotetrazole: 0.28-0.32M
Water (H2O): 1-30ppm

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_f

35.6 °F - (Acetonitrile)

flash_point_c

2 °C - (Acetonitrile)


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