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BM-100

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Auto2D® 2-D Electrophoresis Device

automates 2D gel electrophoresis

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Synonym(s):
2D Electrophoresis, 2DE, IEF, SDS-PAGE

product line

Auto2D®

technique(s)

SDS-PAGE: suitable
electrophoresis: suitable
western blot: suitable

storage temp.

room temp

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This Item
BM-12100BM-12075BM-12065
Auto2D® PAGE Chip 7.5% precast gel, for second-dimension separations on the Auto2D® 2-D Electrophoresis Device.

Millipore

BM-12075

Auto2D® PAGE Chip 7.5%

Auto2D® PAGE Chip 6.5% for second-dimension separations on the Auto2D® 2-D Electrophoresis Device, precast gel

Millipore

BM-12065

Auto2D® PAGE Chip 6.5%

product line

Auto2D®

product line

Auto2D®

product line

Auto2D®

product line

Auto2D®

technique(s)

SDS-PAGE: suitable

technique(s)

-

technique(s)

-

technique(s)

-

General description

The Auto2D® 2-D Electrophoresis Device automates two-dimensional gel electrophoresis. The applied sample enters the first-dimension gel passively, separating proteins by their isoelectric points. After equilibration, the first-dimension gel is set onto a horizontal SDS-PAGE gel, which resolves proteins by molecular weight.

Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requireing advanced user training while offering low reproducibility and high inter-operator variability.

Application

  • Differential proteomic analysis
  • Post-translational modification
  • Protein Isoforms
  • Western Blotting

Features and Benefits

Auto2D® 2-D Electrophoresis Device is user-friendly and enable two-dimensional electrophoresis in 1-2 hours for faster, more reproducible results.

  • Fully automated to eliminate tedious handling steps
  • Fast results in 1-2 hours
  • High reproducibility
  • Easy to use, with no advanced training required
  • Lower inter-operator variability

Storage and Stability

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Auto2D is a registered trademark of Merck KGaA, Darmstadt, Germany

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T1503
Product Number
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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

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Articles

The Auto2D® 2-D Electrophoresis Device fully automates difficult 2D electrophoresis methods in a quick, easy, and reproducible way. Locate difficult-to-find proteins using the Auto2D® system in less than two hours with high reproducibility.

Protocols

The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.

Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.

Related Content

Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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