The sample amount depends on the detection method. The recommended sample amounts are as follows;
CBB staining: 25 µg or more
Fluorescent staining: 25 µg or less
Silver Staining: 10 µg or less
Fluorescent pre-labeling: 3 µg or less
BM-100
Auto2D® 2-D Electrophoresis Device
Automates 2D Gel Electrophoresis from 1st dimension IEF to 2nd dimension SDS-PAGE
Synonym(s):
2D Electrophoresis, 2DE, IEF, SDS-PAGE
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 pkg | Check Cart for Availability | $64,610.00 |
About This Item
NACRES:
NC.01
UNSPSC Code:
41100000
Product Name
Auto2D® 2-D Electrophoresis Device, automates 2D gel electrophoresis
product line
Auto2D®
technique(s)
SDS-PAGE: suitable, electrophoresis: suitable, western blot: suitable
storage temp.
room temp
General description
The Auto2D® 2-D Electrophoresis Device automates two-dimensional gel electrophoresis. The applied sample enters the first-dimension gel passively, separating proteins by their isoelectric points. After equilibration, the first-dimension gel is set onto a horizontal SDS-PAGE gel, which resolves proteins by molecular weight.
Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requireing advanced user training while offering low reproducibility and high inter-operator variability.
Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requireing advanced user training while offering low reproducibility and high inter-operator variability.
Application
- Differential proteomic analysis
- Post-translational modification
- Protein Isoforms
- Western Blotting
Features and Benefits
Auto2D® 2-D Electrophoresis Device is user-friendly and enable two-dimensional electrophoresis in 1-2 hours for faster, more reproducible results.
- Fully automated to eliminate tedious handling steps
- Fast results in 1-2 hours
- High reproducibility
- Easy to use, with no advanced training required
- Lower inter-operator variability
Preparation Note
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Legal Information
Auto2D is a registered trademark of Merck KGaA, Darmstadt, Germany
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| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. - |
| product line Auto2D® | product line Auto2D® | product line Auto2D® | product line Auto2D® |
| technique(s) SDS-PAGE: suitable, western blot: suitable, electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) - |
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.
Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.
Introducción a la PAGE Aprenda sobre el fondo y el protocolo de la SDS-PAGE para la separación de proteínas en función del tamaño en un gel de poliacrilamida.
Articles
El dispositivo de electroforesis 2-D Auto2D® automatiza por completo los métodos de electroforesis bidimensional difíciles de una manera rápida, fácil y reproducible. Localice proteínas difíciles de encontrar utilizando el sistema Auto2D® en menos de dos horas con una gran reproducibilidad.
The Auto2D® 2-DE system automates difficult 2D gel electrophoresis methods for highly reproducible protein analysis in under 2 hours.
Related Content
Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.
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How much is the appropriate sample amount?
1 answer-
Helpful?
-
-
How much is the appropriate sample amount?
1 answer-
The sample amount depends on the detection method. The recommended sample amounts are as follows;
CBB staining: 25 µg or more
Fluorescent staining: 25 µg or less
Silver Staining: 10 µg or less
Fluorescent pre-labeling: 3 µg or lessHelpful?
-