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MilliporeSigma

BM-1RYTJ1

Millipore

Auto2D® Tricine Type Reagent Kit

reagent kit for the Auto2D® 2-D Electrophoresis Device, for better resolution on low molecular weight range

Synonym(s):

2D Electrophoresis, 2DE, 2DE Reagent, Tricine, Tris-Tricine, Tris-Tricine Reagent Kit

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1 KIT
$607.00

$607.00


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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

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product line

Auto2D®

technique(s)

SDS-PAGE: suitable
western blot: suitable

storage temp.

2-8°C

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This Item
BM-1RYSJ1BM-1SPLUXRGTKIT
technique(s)

SDS-PAGE: suitable, western blot: suitable

technique(s)

SDS-PAGE: suitable, western blot: suitable

technique(s)

-

technique(s)

SDS-PAGE: suitable, electrophoresis: suitable, immunoblotting: suitable, western blot: suitable

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

product line

Auto2D®

product line

Auto2D®

product line

Auto2D®

product line

-

General description

Tricine Type Reagent Kit is used for the Auto2D® 2-D Electrophoresis Device with better resolution on low molecular weight range than Glycine Type Reagent Kit.

Application

  • Differential proteomic analysis
  • Post-translational modification
  • Protein Isoforms
  • Western Blotting

Other Notes

  • Rehydration Solution Kit
  • DTT Kit
  • Tricine Type Equilibration Buffer
  • Anode + Buffer
  • Tricine Type Cathode - Buffer

Legal Information

Auto2D is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

El dispositivo de electroforesis 2-D Auto2D® automatiza por completo los métodos de electroforesis bidimensional difíciles de una manera rápida, fácil y reproducible. Localice proteínas difíciles de encontrar utilizando el sistema Auto2D® en menos de dos horas con una gran reproducibilidad.

Auto2D® automates difficult 2D electrophoresis methods for protein localization with high reproducibility in under two hours.

Protocols

The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.

Questions

  1. How much is the appropriate sample amount?

    1 answer
    1. The sample amount depends on the detection method. The recommended sample amounts are as follows;
      CBB staining: 25 µg or more
      Fluorescent staining: 25 µg or less
      Silver Staining: 10 µg or less
      Fluorescent pre-labeling: 3 µg or less

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