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CB1001

Sigma-Aldrich

Anti-GAPDH Mouse mAb (6C5)

liquid, clone 6C5, Calbiochem®

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Synonym(s):
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

6C5, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

frog, human, porcine, chicken, canine, rabbit, mouse, fish, rat

should not react with

bovine, yeast

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)
rabbit ... Gapdh(100009074)

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Anti-GAPDH Antibody from chicken, purified by affinity chromatography

Sigma-Aldrich

AB2302

Anti-GAPDH Antibody

antibody form

purified antibody

antibody form

purified from hybridoma cell culture, purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

6C5, monoclonal

clone

GAPDH-71.1, monoclonal

clone

6C5, monoclonal

clone

polyclonal

form

liquid

form

buffered aqueous solution

form

-

form

-

species reactivity

frog, human, porcine, chicken, canine, rabbit, mouse, fish, rat

species reactivity

bovine, turkey, canine, chicken, monkey, mink, mouse, human, rabbit, rat, hamster

species reactivity

human, feline, pig, mouse, rabbit, fish, canine, rat

species reactivity

human, mouse, horse, rat

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

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General description

Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC.
Protein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.

Immunogen

Rabbit
purified, rabbit muscle

Application


Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Highly Toxic (H)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Analysis Note

Positive Control
Rat skeletal muscle

Other Notes

Useful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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