All Photos(1)



Anti-GAPDH Mouse mAb (6C5)

liquid, clone 6C5, Calbiochem®

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase

Quality Level

biological source


antibody form

purified antibody

antibody product type

primary antibodies


6C5, monoclonal




≤0.1% sodium azide as preservative

species reactivity

fish, chicken, frog, porcine, canine, mouse, rabbit, human, rat

should not react with

yeast, bovine

storage condition

OK to freeze



shipped in

wet ice

storage temp.


General description

Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC.
Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
Protein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.


purified, rabbit muscle


500 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.



Toxicity: Highly Toxic (H)

Physical form

In PBS, pH 7.4.


Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Analysis Note

Positive Control
Rat skeletal muscle

Other Notes

Useful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Karina M Cirone et al.
Cell and tissue research, 379(1), 207-217 (2019-09-04)
Mycobacterium avium subsp. paratuberculosis (MAP) causes chronic diarrheic intestinal infections in domestic and wild ruminants (paratuberculosis or Johne's disease) for which there is no effective treatment. Critical in the pathogenesis of MAP infection is the invasion and survival into macrophages...
Vivek Choudhary et al.
The Journal of investigative dermatology, 135(2), 499-507 (2014-09-19)
Aquaporin-3 (AQP3) is a water and glycerol channel expressed in epidermal keratinocytes. Despite many studies, controversy remains about the role of AQP3 in keratinocyte differentiation. Previously, our laboratory has shown co-localization of AQP3 and phospholipase D2 (PLD2) in caveolin-rich membrane...
Jessica L Binder et al.
PloS one, 15(3), e0230026-e0230026 (2020-03-26)
Pathological accumulation of microtubule associated protein tau in neurons is a major neuropathological hallmark of Alzheimer's disease (AD) and related tauopathies. Several attempts have been made to promote clearance of pathological tau (p-Tau) from neurons. Transcription factor EB (TFEB) has...
David R J Riley et al.
International journal of molecular sciences, 21(1) (2020-01-18)
Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the...
Eric I Elliott et al.
Journal of immunology (Baltimore, Md. : 1950), 200(9), 3047-3052 (2018-04-01)
The NLRP3 inflammasome is activated in response to microbial and danger signals, resulting in caspase-1-dependent secretion of the proinflammatory cytokines IL-1β and IL-18. Canonical NLRP3 inflammasome activation is a two-step process requiring both priming and activation signals. During inflammasome activation...


Loading Controls for Western Blotting

Loading controls in western blotting application.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service