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CB1001

Sigma-Aldrich

Anti-GAPDH Mouse mAb (6C5)

liquid, clone 6C5, Calbiochem®

Synonym(s):
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase

Quality Level

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

6C5, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

fish, chicken, frog, porcine, canine, mouse, rabbit, human, rat

should not react with

yeast, bovine

storage condition

OK to freeze

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

General description

Anti-GAPDH, mouse monoclonal, clone 6C5, recognizes ~146 kDa GAPDH protein under non-reducing conditions & 36 kDa monomers under reducing conditions. It is validated for use in ELISA, WB, and IHC.
Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.
Protein A purified mouse monoclonal antibody. Recognizes the ~146 kDa GAPDH protein under non-reducing conditions and the ~36 kDa monomeric subunits under reducing conditions.

Immunogen

Rabbit
purified, rabbit muscle

Packaging

500 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

Application


Warning

Toxicity: Highly Toxic (H)

Physical form

In PBS, pH 7.4.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.

Analysis Note

Positive Control
Rat skeletal muscle

Other Notes

Useful as a loading or positive control since GAPDH is expressed in all cells. Also reported to work for ELISA and immunocytochemistry. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Karina M Cirone et al.
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The Journal of investigative dermatology, 135(2), 499-507 (2014-09-19)
Aquaporin-3 (AQP3) is a water and glycerol channel expressed in epidermal keratinocytes. Despite many studies, controversy remains about the role of AQP3 in keratinocyte differentiation. Previously, our laboratory has shown co-localization of AQP3 and phospholipase D2 (PLD2) in caveolin-rich membrane...
Jessica L Binder et al.
PloS one, 15(3), e0230026-e0230026 (2020-03-26)
Pathological accumulation of microtubule associated protein tau in neurons is a major neuropathological hallmark of Alzheimer's disease (AD) and related tauopathies. Several attempts have been made to promote clearance of pathological tau (p-Tau) from neurons. Transcription factor EB (TFEB) has...
David R J Riley et al.
International journal of molecular sciences, 21(1) (2020-01-18)
Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the...
Eric I Elliott et al.
Journal of immunology (Baltimore, Md. : 1950), 200(9), 3047-3052 (2018-04-01)
The NLRP3 inflammasome is activated in response to microbial and danger signals, resulting in caspase-1-dependent secretion of the proinflammatory cytokines IL-1β and IL-18. Canonical NLRP3 inflammasome activation is a two-step process requiring both priming and activation signals. During inflammasome activation...

Articles

Loading Controls for Western Blotting

Loading controls in western blotting application.

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