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CBL1512Z

Sigma-Aldrich

Anti-Integrin αM [CD11b] Antibody, clone OX-42

clone OX-42, Chemicon®, from mouse

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Synonym(s):
CD11b
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

OX-42, monoclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

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clone

OX-42, monoclonal

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OX-42, monoclonal

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OX-42, monoclonal

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ICRF44, monoclonal

Gene Information

rat ... Itgam(25021)

Gene Information

rat ... Itgam(25021)

Gene Information

rat ... Itgam(25021)

Gene Information

human ... ITGAM(3684)

species reactivity

rat

species reactivity

rat

species reactivity

rat

species reactivity

human

antibody form

affinity purified immunoglobulin

antibody form

purified antibody

antibody form

-

antibody form

purified immunoglobulin

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

200

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Specificity

This antibody recognizes most macrophages (including resident peritoneal and activated macrophages), Kupffer cells, but only about 35% of alveolar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95 KDa. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3. FUSION PARTNER: NSO myeloma cell line.

Immunogen

Rat peritoneal macrophages

Application

Detect Integrin αM [CD11b] using this Anti-Integrin αM CD11b Antibody, clone OX-42 validated for use in FC, IH, IH(P) & IP.
Flow cytometry

Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15′ no post-fix).

Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG bead for capture only. The use of protein A or protein G is not recommended.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Linkage

Replaces: MAB1405Z

Physical form

Format: Purified
The immunoglobulin was purified by protein G affinity chromatography and is presented as a liquid in phosphate buffered saline containing no preservatives.

Storage and Stability

Maintain in undiluted aliquots at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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