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Anti-Vimentin Antibody, clone VIM 3B4

clone VIM 3B4, Chemicon®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

VIM 3B4, monoclonal

species reactivity

chicken, amphibian, bovine, monkey, canine, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... VIM(7431)

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MAB1687MABT121SAB4700524
clone

VIM 3B4, monoclonal

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43BE8, monoclonal

clone

3CB2, monoclonal

clone

VI-RE/1, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

chicken, amphibian, bovine, monkey, canine, human

species reactivity

human, monkey

species reactivity

chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard

species reactivity

human

Gene Information

human ... VIM(7431)

Gene Information

human ... VIM(7431)

Gene Information

human ... VIM(7431)

Gene Information

human ... VIM(7431)

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

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Specificity

Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.

Immunogen

Vimentin purified from bovine lens

Application

Western blot
Immunohistochemistry: 1:100; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
Cytoskeleton
This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.

Target description

57 kDa

Physical form

Format: Purified
Protein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Protein A purified

Storage and Stability

Maintain at 2–8°C for up to 12 months from date of receipt.

Analysis Note

Control
Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


Certificates of Analysis (COA)

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?-fetoprotein involvement during glucocorticoid-induced precocious maturation in rat colon.
Chen M, Sun P, Liu XY, Dong D, Du J, Gu L, Ge YB
World Journal of Gastroenterology null
W Bohn et al.
Experimental cell research, 201(1), 1-7 (1992-07-01)
Two commercially available monoclonal antibodies raised against the intermediate filament protein vimentin were characterized concerning their species-specific reaction pattern on vertebrate cells. The antibody V9 exhibited extensive reactivity with vimentin of all mammalian species tested, but specifically did not detect
K R Rogers et al.
European journal of cell biology, 66(2), 136-150 (1995-02-01)
We have investigated the effect of stepwise truncating the carboxyterminal domain ("tail") of the intermediate filament (IF) protein vimentin of the clawed toad, Xenopus laevis, on filament assembly in vitro and, using cell transfection, in vivo and also on the
Dynamic epigenetic regulation of the microRNA-200 family mediates epithelial and mesenchymal transitions in human tumorigenesis.
V Davalos,C Moutinho,A Villanueva,R Boque,P Silva,F Carneiro,M Esteller
Oncogene null
Cytoskeletal components of lymphoid organs. I. Synthesis of cytokeratins 8 and 18 and desmin in subpopulations of extrafollicular reticulum cells of human lymph nodes, tonsils, and spleen.
Franke, W W and Moll, R
Differentiation, 36, 145-163 (1987)

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