Unfortunately, this product has not been validated for use in Western Blot.
CBL271
Anti-Fibroblasts Antibody, clone TE-7
clone TE-7, Chemicon®, from mouse
Synonym(s):
Fibroblast Marker
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100 μG
$476.00
$476.00
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A recombinant, preservative-free antibody is available for your target. Try ZMS1048
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
TE-7, monoclonal
Species reactivity:
human
Application:
FACS, ICC, IF, IHC
Citations:
34
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biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
TE-7, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FGF1(2246)
Related Categories
1 of 4
This Item | |||
|---|---|---|---|
| antibody form purified antibody | antibody form purified immunoglobulin | antibody form purified antibody | antibody form purified immunoglobulin |
| species reactivity human | species reactivity bovine, human | species reactivity human | species reactivity human |
| Gene Information human ... FGF1(2246) | Gene Information human ... FGF2(2247) | Gene Information - | Gene Information human ... ITGAV(3685) |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| clone TE-7, monoclonal | clone FB-8, monoclonal | clone TE-7, recombinant monoclonal | clone E7P6, monoclonal |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Immunogen
Whole human thymic stoma cells (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168).
Application
Immunohistochemistry Analysis: A representative lot immunostained fibroblastic cells in paraffin-embedded gastrocnemius section from a patient with myopathic condition (Courtesy of Duy Minh Ha, University of Nebraska Medical Center, Omaha, NE)
Immunocytochemistry Analysis: A representative lot immunostained human muscle fibroblasts by fluorescent immunocytochemistry (Agley, C.C., et al. (2015). J. Vis. Exp. (95):52049).
Immunocytochemistry Analysis: A representative lot immunostained MRC-5 fetal fibroblasts and normal human dermal fibroblasts (NHDF), but not fibrocytes or macrophages by immunocytochemistry (Bertolotto, C., et al. (2009). PLoS One. 4(10):e7475).
Flow Cytometry Analysis: A representative lot detected fibroblasts differentiated from human ESCs and iPSCs (Zhang, J., et al. (2012). Circ. Res. 111(9):1125-1136).
Immunofluorescence Analysis: A representative lot immunostained fibroblasts in human skeletal muscle cryosections by fluorescent immunohistochemistry (Agley, C.C., et al. (2013). J. Cell. Sci. 126(Pt 24):5610-5625).
Immunofluorescence Analysis: A representative lot immunostained the fibrous stroma and vessels in acetone-fixed, frozen human tissue sections by fluorescent immunohistochemistry (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168).
Immunocytochemistry Analysis: A representative lot immunostained human muscle fibroblasts by fluorescent immunocytochemistry (Agley, C.C., et al. (2015). J. Vis. Exp. (95):52049).
Immunocytochemistry Analysis: A representative lot immunostained MRC-5 fetal fibroblasts and normal human dermal fibroblasts (NHDF), but not fibrocytes or macrophages by immunocytochemistry (Bertolotto, C., et al. (2009). PLoS One. 4(10):e7475).
Flow Cytometry Analysis: A representative lot detected fibroblasts differentiated from human ESCs and iPSCs (Zhang, J., et al. (2012). Circ. Res. 111(9):1125-1136).
Immunofluorescence Analysis: A representative lot immunostained fibroblasts in human skeletal muscle cryosections by fluorescent immunohistochemistry (Agley, C.C., et al. (2013). J. Cell. Sci. 126(Pt 24):5610-5625).
Immunofluorescence Analysis: A representative lot immunostained the fibrous stroma and vessels in acetone-fixed, frozen human tissue sections by fluorescent immunohistochemistry (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Anti-Fibroblasts Antibody, clone TE is validated for use in Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF) and Immunohistochemistry (IHC) for the detection of Fibroblasts.
Biochem/physiol Actions
The antibody reacts with fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen defined by antibody TE-7 appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue, e.g. fibrosarcomas (Haynes, B.F., et al. (1984). J. Exp. Med. 159(4):1149-1168). The antibody is useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.
Physical form
Format: Purified
The monoclonal is presented at a concentration of 100 μg/mL in phosphate buffered saline containing 10 mM sodium azide and 1 mg/mL bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Preparation Note
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK 2
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flash_point_c
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Cecilie J L Bechshøft et al.
Journal of applied physiology (Bethesda, Md. : 1985), 127(2), 342-355 (2019-05-24)
Underpinning skeletal muscle plasticity is the interplay between many cell types, of which fibroblasts are emerging as potent players, both negatively in the development of fibrosis but also positively in stimulating muscle repair through enhancing myogenesis. The mechanisms behind this
Eliana Steinberg et al.
International journal of molecular sciences, 21(20) (2020-10-22)
The currently accepted imaging methods have been a central hurdle to imaging the finer details of tumor behavior in three-dimensional (3D) ex vivo multicellular culture models. In our search for an improved way of imaging tumor behavior in its physiological-like
Katharine M Hinchcliff et al.
Journal of plastic, reconstructive & aesthetic surgery : JPRAS, 70(5), 568-576 (2017-03-28)
AlloDerm RTU® and AlloMaxTM are two acellular dermal matrices (ADMs) used in implant-based breast reconstruction. In this study, we examined whether different processing methods for the ADMs lead to a disparity in histologic, clinical, and financial outcomes after breast reconstruction.
Duy M Ha et al.
Journal of translational medicine, 14, 39-39 (2016-02-06)
Lower leg ischemia, myopathy, and limb dysfunction are distinguishing features of peripheral artery disease (PAD). The myopathy of PAD is characterized by myofiber degeneration in association with extracellular matrix expansion, and increased expression of transforming growth factor-beta 1 (TGF-β1; a
B N Napiwocki et al.
Physiological reports, 9(19), e15045-e15045 (2021-10-08)
In native heart tissue, cardiac fibroblasts provide the structural framework of extracellular matrix (ECM) while also influencing the electrical and mechanical properties of cardiomyocytes. Recent advances in the field of stem cell differentiation have led to the availability of human
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