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CC117

Sigma-Aldrich

Chicken Extracellular Chondroitin Sulfate Proteoglycans

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eCl@ss:
32160405
NACRES:
NA.75

biological source

chicken

Quality Level

100
300

assay

95%

form

liquid

mol wt

<400 kDa

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

chicken ... VCAN(395565)

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H4777C7786A5503
vibrant-m

H4777

Heparan sulfate proteoglycan

vibrant-m

C7786

Conalbumin from chicken egg white

vibrant-m

A5503

Albumin from chicken egg white

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

bioconjugation: suitable

assay

95%

assay

-

assay

-

assay

≥98% (agarose gel electrophoresis)

Quality Level

100, 300

Quality Level

300

Quality Level

200

Quality Level

300

biological source

chicken

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

biological source

chicken egg white

biological source

chicken egg white

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

shipped in

-

General description

Aggrecan, versican, and neurocan are members of the family of hyaluronic acid-binding proteoglycans and therefore each contains hyaluronic acid binding regions, Ig-like domains, EGF like domains, lectin-like domains, and complement regulatory-like domains. Phosphacan is distinct; it contains a region of similarity to carbonic anhydrase, a fibronectin type III domain, and is a splice variant of the transmembrane protein receptor tyrosine phosphatase equivalent to its extracellular portion.

Although aggrecan was traditionally thought of as a cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four of these proteoglycans have broad yet distinctive distribution patterns inearly embryos. The presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and are capable of inhibiting both cell-ECM and cell-cell adhesion.
DESCRIPTION/COMPOSITION:CC117 contains a mixture of large, extracellular chondroitin sulfateproteoglycans isolated from embryonic chicken brain. Amino acid sequence analyses and immunological studies have demonstrated that the major components of this mixture are neurocan, phosphacan, versican, and aggrecan. Following chondroitinase treatment to remove the glycosaminoglycan portion of these proteoglycans, the major components of this preparation resolved on SDS gels are 250 kDa core proteins representing neurocan and 400 kDa core proteins representing aggrecan, phosphacan, and versican.

*Quantity: Protein as determined using the bicinchoninic acid (BCA) assay. Note that 100 μg is a measurement of core polypeptides. Components of this mixture contain core polypeptide plus attached glycosaminoglycans
Product Source: Isolated from whole 12-14 day old embryonic chicken brains.

Application

CC117 has been used successfully with rat and mouse brain slice culture systems.

Physical form

Provided as a liquid in PBS, containing no preservatives.

Storage and Stability

Store at -20°C for up to 12 months. Avoid repeated freeze/thaw.

Analysis Note

Mixture of large, extracellular chondroitin sulfate proteoglycans (CSPGs) purified from embryonic chicken brains by chromatrographic methods. By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of each CSPG contained in the mixture is not quantified, however the virtually all of the CSPGs present in the source brains are collected.

HOMOGENEITY:

By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of CSPG species has not been determined.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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