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CC118

Sigma-Aldrich

Chicken Tenascin

Synonym(s):

Cytotactin, Tenascin-C

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77

biological source

chicken

Quality Level

form

liquid

mol wt

large molecular weight polypeptide 190 kDa
large molecular weight polypeptide 200 kDa
large molecular weight polypeptide 220 kDa

manufacturer/tradename

Chemicon®

concentration

0.5 mg/mL

technique(s)

cell culture | mammalian: suitable

input

sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type epithelial cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

chicken ... CNTN5(395317)

General description

Product Source: Purified from embrionic chicken brains.
Tenascin is a large glycoprotein that forms disulfide-linked hexamers. It is made up of repeated structural domains including EGF-like repeats, fibronectin type III repeats, and a region homologous to fibrinogen. Splice variants of tenascin differ in their number of fibronectin type III repeats. Tenascin is expressed in distinctive spatial and temporal patterns in most embryonic tissues at sites of tissue remodeling and cell migration. There is little expression of tenascin in adult tissues except, again, at sites of tissue remodeling and cell migration such as surrounding tumors, in healing wounds, and in branching or damaged blood vessels. Tenascin binds to several cell-surface receptors and mediates cell-substrate adhesion. Nevertheless, tenascin is frequently referred to as an anti-adhesion molecule because for a variety of cell types the presence of tenascin inhibits adhesion to other ECM proteins present. This effect occurs because the binding of tenascin to certain receptors inhibits cell spreading and thereby blocks the stabilization of adhesion that results from cell preading in vitro assays.

Physical form

Provided as a liquid in PBS, containing no preservatives.

Storage and Stability

Store at -20°C for up to 12 months. Avoid repeated freeze/thaw.

Analysis Note

Isolated from embryonic chicken brains by chromatographic methods. Contains three characteristic polypeptide chains (220, 200, and 190 kDa) that migrate on SDS gels as a high molecular weight multimer under non-reducing conditions.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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J Spring et al.
Cell, 59(2), 325-334 (1989-10-20)
A structural and functional model of tenascin was elaborated using recombinant parts of three alternatively spliced tenascin variants and anti-tenascin monoclonal antibodies. The fusion proteins were compared with intact tenascin for their functions and by electron microscopy. A strong cell

Protocols

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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