CCW4115-50X2ML

Yeast Extract Broth

tube capacity 3 mL, filling volume 2 mL, ready-to-use, box of 50 tubes

• Synonym(s): YEB, Yeast Peptone Dextrose Broth
• UNSPSC Code: 41106217

Properties

• agency: EP 2.6.1; JP 4.06; USP 71
• form: liquid
• shelf life: 9 mo.
• feature: ready-to-use
• packaging: box of 50 tubes
• technique(s): microbiological culture: suitable
• filling volume: 2 mL
• tube capacity: 3 mL
• application(s): cosmetics; food and beverages; pharmaceutical
• components: : 6 g/L (Tryptone ); : 3 g/L (Yeast )
• shipped in: 2-8°C
• Storage temp.: 2-8°C

Description

General description

Yeast Extract Broth is a water-soluble, peptone-like derivative obtained from yeast (Saccharomyces) cells. It is prepared through the proteolytic autolysis of the cells, with the autolytic activity being arrested by a heating process. The controlled autolysis ensures the preservation of naturally occurring B-complex vitamins. Yeast Extract is a versatile, growth-enhancing nutritive substrate that can replace serum in cell cultures. And, it maintains an endotoxin value of less than 500 EU/g.

Application

Yeast Extract Broth is used to detect microorganisms from water samples.

Analysis Note

• Utilize the membrane filtration method and impregnate a sterile pad with the medium
• Deposit the membrane filter on the pad and ensure protection against dehydration
• Adjust temperature and incubation time as per internal regulations or laboratory methodologies
• Collect, dilute, and prepare samples following specifications, directives, official standard regulations, or expected results
• Incubate aerobically at 36±2ºC for 44±4h
• Note that different incubation times or temperatures may be required based on the sample or normative ISO factors
• Enumerate all colonies on the membrane surface post-incubation
• Recognize the significant impact of the material and quality of the membrane filter on microorganism recovery

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable