DR1047

Anti-UHRF1 Mouse mAb (3A11)

liquid, clone 3A11, Calbiochem^®

• Synonym(s): Anti-Ubiquitin-Like PHD/RING Finger Domain 1

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3A11, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... UHRF1(29128)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~90 kDa UHRF1 protein.

Recognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.

This Anti-UHRF1 Mouse mAb (3A11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of UHRF1.

Immunogen

A recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein

NA

Application

ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)

Warning

Toxicity: Regulatory Review (Z)

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
HeLa cells, Human endometrium tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Mistry H, et al. 2008. Biochem. Biophys. Res. Commun.375 321.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable