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ECM815

Sigma-Aldrich

Osteogenesis Quantitation Kit

The Osteogenesis Quantitation Kit provides all the necessary reagents & a protocol to enable quantification of osteogenesis using a standard plate reader.

eCl@ss:
32161000

Quality Level

manufacturer/tradename

Chemicon®

technique(s)

cell based assay: suitable

detection method

colorimetric

shipped in

wet ice

General description

CHEMICON′s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation can be facilitated by use of this kit.

The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. Enough reagents are provided to perform 48 quantitation assays on 24 well plates.

For Research Use Only. Not for use in diagnostic procedures.

Application

Note: Kit componenet 10X ARS Dilution Buffer: Part No. 2004810
One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.
Research Category
Cell Structure

Components

Alizarin Red S Stain Solution (1X): Part No. 2003999, One bottle containing 50 mL Alizarin Red S Solution. Store at 2-8°C.

10% Acetic Acid: Part No. 2004807, One bottle containing 20 mL of 10% Acetic Acid in deionized water. Store at 2-8°;C.

10% Ammonium Hydroxide: Part No. 2004809, One bottle containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2-8°C.

10X ARS Dilution Buffer: Part No. 2004810, One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. Store at 2-8°C.

Storage and Stability

All components should be stored at 2 - 8ºC until expiration date printed on label.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Nadja Engel et al.
Scientific reports, 11(1), 22228-22228 (2021-11-17)
A clinical implementation of cell-based bone regeneration in combination with scaffold materials requires the development of efficient, controlled and reproducible seeding procedures and a tailor-made bioreactor design. A perfusion system for efficient, homogeneous, and rapid seeding with human adipogenic stem
Florian Andreas Probst et al.
Scientific reports, 10(1), 2062-2062 (2020-02-08)
Reconstruction of bone defects represents a serious issue for orthopaedic and maxillofacial surgeons, especially in extensive bone loss. Adipose-derived mesenchymal stem cells (ADSCs) with tri-calcium phosphates (TCP) are widely used for bone regeneration facilitating the formation of bone extracellular matrix
Mona Alsulaiman et al.
Journal of cell communication and signaling, 10(1), 17-31 (2015-12-03)
Lysyl oxidase pro-enzyme is secreted by tumor cells and normal cells as a 50 kDa pro-enzyme into the extracellular environment where it is cleaved into the ~30 kDa mature enzyme (LOX) and 18 kDa pro-peptide (LOX-PP). Extracellular LOX enzyme activity is required for
Xiang Fang et al.
PloS one, 9(2), e88874-e88874 (2014-03-04)
A number of publications have reported that adipose derived stem cells (ADSCs) have the capacity to be induced to differentiate into osteoblasts both in vitro and in vivo. However, it has been difficult to use separate ADSCs for cortical bone
Jifeng Xu et al.
Aging, 13(15), 19282-19292 (2021-08-11)
Osteoporosis is a common aging-related disease diagnosed primarily using bone mineral density (BMD). Extracellular vesicles (EVs) remain unexplored in the context of osteoporosis. Towards this, EVs were isolated from plasma of a discovery cohort with 8 non-osteoporotic and 8 osteoporotic

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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