The assay detects GLP-1 (1-37, 1-36, 7-37), with a moderate affinity and exhibits 12-15% cross-reactivity.
EZGLP1T
Multi Species GLP-1 ELISA Kit
measures total GLP-1 levels in 20 µl - 50 µl serum, plasma or cell culture supernatent
Synonym(s):
Glucagon-like peptide-1
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EZGLP1T-36K
$772.00
EZGLP1T-36BK
$5,370.00
About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
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Product Name
Multi Species GLP-1 Total ELISA, EZGLP1T-36K, This Multi Species GLP-1 Total ELISA, EZGLP1T-36K, is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.
Quality Level
species reactivity
human, mouse, rat
packaging
kit of 1 × 96 wells
parameter
20—50 μL sample volume (4hr assay)
assay range
linearity: 90-132%
recovery range: 90-110%
sensitivity: 1.5 pM
(MinDC)
standard curve range: 4.1-1000 pM
inter-assay cv: <12%
intra-assay cv: <5%
technique(s)
ELISA: suitable
input
sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum
NCBI accession no.
UniProt accession no.
application(s)
research use
detection method
colorimetric (450nm/590nm)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... GCG(2641)
1 of 4
This Item | EZRMGIP | RAB0671 | EZRGRT |
|---|---|---|---|
| Gene Information human ... GCG(2641) | Gene Information - | Gene Information human ... GCG(2641) | Gene Information - |
| species reactivity human, mouse, rat | species reactivity mouse, rat | species reactivity human | species reactivity mouse, rat |
| input sample type cell culture supernatant | input sample type cell culture supernatant | input sample type plasma | input sample type plasma (K2 EDTA) |
| assay range linearity: 90-132%, sensitivity: 1.5 pM | assay range - | assay range - | assay range - |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable |
General description
Glucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.
Application
Research Category
Metabolism
Metabolism
This is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.
Used to detect/quantify: Glucagon Like Peptide-1
Biochem/physiol Actions
Cross Reactivty
GLP-1 is conserved in almost all mammals.
GLP-1 is conserved in almost all mammals.
The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.
Disclaimer
For research use only. Not for use in diagnostic procedures.
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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Yasutaka Takeda et al.
Physiological reports, 8(11), e14469-e14469 (2020-05-31)
The short-form glucose-dependent insulinotropic polypeptide (GIP) (1-30) is released from islet alpha cells and promotes insulin secretion in a paracrine manner in vitro. However, it is not well elucidated how GIP (1-30) is involved in glucose metabolism in vivo, since
Lindsey M Rings et al.
Journal of veterinary internal medicine, 33(6), 2752-2764 (2019-10-31)
The enteroinsular axis (EIA) comprises intestinal factors (incretins) that stimulate insulin release after PO ingestion of nutrients. Glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are the main incretins. The EIA has not been investigated in healthy neonatal foals but
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What is the cross reactivity to other forms of GLP-1?
1 answer-
Helpful?
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Are there any instances of cross-reactivity to Liraglutide when testing patient samples treated with Liraglude in EZGLP1T-39K, considering that these patient samples read higher than control samples?
1 answer-
The kit has not been tested for cross-reactivity to liraglutide. Since liraglutide is similar to GLP-1 7-37 and our kit has 15% cross-reactivity to GLP-1 7-37, there is a possibility of some cross-reactivity.
Helpful?
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What information is available for the antibodies used in the GLP-1 Total ELISA kit (EZGLP1T-36K)?
1 answer-
The sequence and epitope are proprietary. The capture involves a polyclonal rabbit anti GLP-1 total, while the detection uses a monoclonal mouse anti GLP-1 (7-36), and the immunogen is mid molecule.
Helpful?
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