Yes, the kit is compatible with cell/tissue culture supernatant samples. In this case, the user would omit the Matrix Solution and replace it with their blank culture media in the background, standard, and QC wells. While the samples can contain a DPP-IV inhibitor, it is not a requirement. It is recommended to follow the same sample conditions throughout the study.
EZGLP2
Rat/Human GLP-2 ELISA Kit
detects GLP-2 in 50 μL serum, plasma or cell culture supernatent
Synonym(s):
Glucagon-like peptide-2
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| Size/SKU | Availability | Price |
|---|---|---|
96 wells | Estimated to ship TODAY | $772.00 |
About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Input:
sample type plasma (K2 EDTA)
sample type serum
sample type serum
Species reactivity:
human, rat
Storage temp.:
2-8°C
Shipped in:
wet ice
Product Name
GLP-2 ELISA, This GLP-2 ELISA is used to measure & quantify GLP-2 levels in Metabolism & Endocrine research.
Quality Segment
species reactivity
human, rat
packaging
kit of 1 × 96 wells
parameter
50 μL sample volume (4hr assay)
technique(s)
ELISA: suitable
input
sample type plasma (K2 EDTA)
sample type serum
application(s)
research use
assay range
accuracy: 75-97%
(Human), accuracy: 94-109%
(Rat), sensitivity: 1 ng/mL
(50 μl sample size), standard curve range: 1-64 ng/mL, inter-assay cv: <12%
intra-assay cv: <10%
detection method
colorimetric (450nm/590nm)
shipped in
wet ice
storage temp.
2-8°C
General description
The glucagon-like peptides GLP-1 and GLP-2 are important gut hormones released from the small intestine upon nutrient ingestion. In circulation, GLP-2 is present in two molecular forms, GLP-2 (1-33) and GLP-2 (3-33). Similar to GLP-1, active form GLP-2 (1-33) is cleaved to inactive form GLP-2 (3-33) by protease DPPIV. GLP-2 has been shown to play important roles in the regulation of gastrointestinal functions (digestion, absorption, motility, epithelial growth, and blood flow) and bone resorption. Thus, GLP-2 or its analogs may have therapeutic potentials for the treatment of diseases such as Crohn’s disease and osteoporosis.
Application
Research Category
Metabolism
Metabolism
Research Sub Category
Osteoporosis
Osteoporosis
This assay requires 50 µl of rat or human samples and is performed at room temperature as a 4 hour assay. GLP-2 has also been detected in guinea pig, horse, porcine, and non-human primate samples.
Used to detect/quantify: GLP-2
Biochem/physiol Actions
Human GLP-2 1-33 100%
Human GLP-2 3-33 112%
Rat GLP-2 101%
Human GLP-2 3-33 112%
Rat GLP-2 101%
Other Notes
Please contact Technical Service for linearity of dilution.
Disclaimer
For research use only. Not for use in diagnostic procedures.
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This Item | |||
|---|---|---|---|
| species reactivity human, rat | species reactivity human, mouse, rat | species reactivity human | species reactivity mouse, rat |
| input sample type plasma (K2 EDTA) | input sample type cell culture supernatant | input sample type plasma (K2 EDTA) | input sample type plasma (K2 EDTA) |
| assay range accuracy: 75-97% | assay range - | assay range accuracy: 101-107% | assay range accuracy: 86-87% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| detection method colorimetric (450nm/590nm) | detection method colorimetric (450nm/590nm) | detection method colorimetric (450nm/590nm) | detection method colorimetric (450nm/590nm) |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable |
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
Certificates of Analysis (COA)
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Is the kit compatible with cell/tissue culture supernatants that contain a DPP-IV inhibitor, considering that the same sample will be used to measure GLP-1 active?
1 answer-
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