The GLP-1 antibodies are specific to the active form. The cross-reactivity to 7-36 is 100% and the cross-reactivity to 7-37 is 72%. There is no reactivity to the 9-36 form.
EZGLPHS
Multi Species GLP-1 ELISA Kit
measures active GLP-1 levels in 50 μL plasma
Synonym(s):
GLP-1 Active ELISA Kit, GLP-1 ELISA kit
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.84
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Product Name
High Sensitivity GLP-1 Active ELISA Kit, Chemiluminescent,
species reactivity
mouse, rat, human
Quality Level
packaging
kit of 1 × 96 wells
parameter
50 μL sample volume (4hr assay)
technique(s)
ELISA: suitable
input
sample type plasma (K2 EDTA)
sample type serum
application(s)
research use
assay range
inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 0.14 pM
standard curve range: 0.14 -100 pM
accuracy: 96%
detection method
chemiluminescent (425nm)
shipped in
wet ice
storage temp.
2-8°C
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This Item | EZGLU | EZGLP1T | RAB0055 |
|---|---|---|---|
| species reactivity mouse, rat, human | species reactivity porcine, human, mouse, rat | species reactivity human, mouse, rat | species reactivity mouse |
| assay range inter-assay cv: <15% | assay range inter-assay cv: <10% | assay range - | assay range inter-assay cv: <12% |
| input sample type plasma (K2 EDTA) | input sample type plasma (K2 EDTA) | input sample type cell culture supernatant | input sample type serum |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| detection method chemiluminescent (425nm) | detection method chemiluminescent (425nm) | detection method colorimetric (450nm/590nm) | detection method colorimetric |
| technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable | technique(s) ELISA: suitable, capture ELISA: suitable |
General description
This high sensitivity GLP-1 Active ELISA kit is used for the quantification of GLP-1 (7-36) in serum, plasma and cell culture samples. The GLP-1 sequence is highly conserved between the species, with no sequence variation occurring in mammals. One kit is sufficient to measure 38 unknown samples in duplicate.
Specificity
GLP-1 (7-36) 100%
GLP-1 (7-37) 72%
GLP-1 (1-36) <3%
GLP-1 (1-37) <2%
Used to detect/quantify: GLP-1 (Active)
GLP-1 (7-37) 72%
GLP-1 (1-36) <3%
GLP-1 (1-37) <2%
Used to detect/quantify: GLP-1 (Active)
Application
This High Sensitivity GLP-1 Active ELISA Kit, Chemiluminescent is used to measure & quantify GLP-1 (Active) levels in Metabolism & Neuroscience research.
Research Category: Metabolism
Research Sub-Category: Obesity, Metabolic Disorders
Research Category: Metabolism
Research Sub-Category: Obesity, Metabolic Disorders
Other Notes
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Disclaimer
For research use only. Not for use in diagnostic procedures.
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Is the Multi Species GLP-1 ELISA Kit (EZGLPHS) specific to the active form of GLP-1 (7-36) or does it reconize the amide form (9-36) ? Thanks
1 answer-
Helpful?
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Does this work with mouse plasma? If so, how many ul of plasma will I need? Thanks
1 answer-
The GLP-1 sequence is highly conserved among mammals, allowing the GLP-1 ELISA to be used with mouse samples. The sample volume is 50 ul per well and it is recommended to run samples in duplicate.
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