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EZIL6

Sigma-Aldrich

Human IL-6 Conferma ELISA

quantification of human IL-6 in serum, plasma, or culture media samples

Synonym(s):
IL6

species reactivity

human

assay range

linearity: 105-116%
(serum)

linearity: 88-105%
(plasma)

inter-assay cv: <15%
intra-assay cv: <5%
standard curve range: 1.17-300 pg/mL
accuracy: 91-109%

technique(s)

ELISA: suitable

input

sample type serum
sample type culture(s)
sample type plasma

UniProt accession no.

detection method

colorimetric

Gene Information

human ... IL6(3569)

General description

This Human Interleukin-6 (IL-6) Conferma ELISA kit is used for the quantification of Human IL-6 in serum, plasma, or culture media samples. Human IL-6 is a soluble protein cytokine most commonly associated with the acute inflammatory response to infection or tissue damage.

This is a 96 well format sandwich ELISA which uses our Conferma process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots. For detailed information on the process and a Verification report for this assay please click hereConferma™ Sandwich ELISAs

Entrez Gene Summary:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections.

UniProt Summary:
  • Function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism.
  • Size: 212 amino acids; 23,718 Da
  • Subunit: Component of a hexamer of two molecules each of IL6, IL6R and IL6ST.
  • Subcellular location: Extracellular region or secreted
  • Disease: Rheumatoid arthritis systemic juvenile (RASJ). A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Similarity: Belongs to the IL-6 superfamily.

Application

One kit is sufficient to measure 37 samples in duplicate

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Analysis Note

3 hour 45 minute assay, room temperature, 50 μL sample size (serum, plasma, or tissue culture medium)

Legal Information

CONFERMA is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

Certificate of Analysis

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Certificate of Quality

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