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EZIL6
Human IL-6 Conferma™ ELISA
quantification of human IL-6 in serum, plasma, or culture media samples
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Synonym(s):
IL6
species reactivity
human
assay range
linearity: 105-116%
(serum)
linearity: 88-105%
(plasma)
inter-assay cv: <15%
intra-assay cv: <5%
standard curve range: 1.17-300 pg/mL
accuracy: 91-109%
technique(s)
ELISA: suitable
input
sample type serum
sample type culture(s)
sample type plasma
UniProt accession no.
detection method
colorimetric
Gene Information
human ... IL6(3569)
General description
This Human Interleukin-6 (IL-6) Conferma™ ELISA kit is used for the quantification of Human IL-6 in serum, plasma, or culture media samples. Human IL-6 is a soluble protein cytokine most commonly associated with the acute inflammatory response to infection or tissue damage.
This is a 96 well format sandwich ELISA which uses our Conferma™ process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots. For detailed information on the process and a Verification report for this assay please click hereConferma™ Sandwich ELISAs
Entrez Gene Summary:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections.
UniProt Summary:
This is a 96 well format sandwich ELISA which uses our Conferma™ process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots. For detailed information on the process and a Verification report for this assay please click hereConferma™ Sandwich ELISAs
Entrez Gene Summary:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections.
UniProt Summary:
- Function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism.
- Size: 212 amino acids; 23,718 Da
- Subunit: Component of a hexamer of two molecules each of IL6, IL6R and IL6ST.
- Subcellular location: Extracellular region or secreted
- Disease: Rheumatoid arthritis systemic juvenile (RASJ). A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
- Similarity: Belongs to the IL-6 superfamily.
Application
One kit is sufficient to measure 37 samples in duplicate
Research Category:
Research Category:
- Cytokines
- Inflammation & Immunology
- Immune Response
- Metabolic Disease
Analysis Note
3 hour 45 minute assay, room temperature, 50 μL sample size (serum, plasma, or tissue culture medium)
Legal Information
CONFERMA is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
For research use only. Not for use in diagnostic procedures.
Certificate of Analysis
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Certificate of Quality
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