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EZMCP1

Sigma-Aldrich

Human MCP-1 Conferma ELISA

quantification of human MCP-1 in serum, plasma, or culture media samples.

clone

monoclonal

species reactivity

human

assay range

linearity: 94-106 (serum)%
linearity: 94-110 (plasma)%
inter-assay cv: <12.9%
intra-assay cv: <4.9%
standard curve range: 1.17-300 pg/mL
accuracy: 84-95%

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma
sample type culture(s)

UniProt accession no.

detection method

colorimetric

Gene Information

human ... CCL2(6347)

General description

Human MCP-1 is a chemokine assoscited with the attraction of Monocytes and Basophils to the site of a pathology. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. In particilar this is a marker of inflammatory disorders. It binds to chemokine receptors CCR2 and CCR4.
This is a 96 well format sandwich ELISA which uses our Conferma process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human MCP-1 Conferma ELISA kit is used for the quantification of Human MCP-1 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Analysis Note

3 hour 45 minute assay, room temperature, 1:5 dilution recommended e.g.30 μL sample + 120μL of diluent (serum, plasma, or tissue culture medium)

Legal Information

CONFERMA is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

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Certificate of Quality

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