EZRMI

Rat/Mouse Insulin ELISA Kit

Name: Rat/Mouse Insulin ELISA Kit
Brand: MM

measures and quantifies insulin levels in 10 μL serum or plasma

Synonym(s):

Insulin

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About This Item

UNSPSC Code:

12161503

eCl@ss:

32161000

NACRES:

NA.32

Key Documents

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Product Name

Rat/Mouse Insulin ELISA, This Rat/Mouse Insulin ELISA is used to measure & quantify Insulin levels in Metabolism & Endocrine research.

Quality Level

200

species reactivity

mouse, rat

packaging

kit of 1 × 96 wells

parameter

10 μL sample volume (4hr assay)

assay range

accuracy: 83-102%
linearity: 79-133%
sensitivity: 0.2 ng/mL
standard curve range: 0.2-10 ng/mL

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma (K2 EDTA)

UniProt accession no.

P01322
P01325

application(s)

research use

detection method

colorimetric (450nm/590nm)

shipped in

wet ice

storage temp.

2-8°C

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This Item	EZHI	EZRMCP2	EZRMGIP
Millipore EZRMI Rat/Mouse Insulin ELISA	Millipore EZHI Human Insulin ELISA	Millipore EZRMCP2 Rat/Mouse C-Peptide 2 ELISA	Millipore EZRMGIP Rat/Mouse GIP (total) ELISA
species reactivity mouse, rat	species reactivity human	species reactivity mouse, rat	species reactivity mouse, rat
input sample type serum sample type plasma (K2 EDTA)	input sample type plasma (K2 EDTA) sample type serum sample type cell culture supernatant	input sample type plasma (K2 EDTA) sample type serum	input sample type cell culture supernatant sample type plasma (K2 EDTA) sample type serum
assay range accuracy: 83-102%, sensitivity: 0.2 ng/mL, linearity: 79-133%, standard curve range: 0.2-10 ng/mL	assay range accuracy: 76-103%, linearity: 96-112%, standard curve range: 2-200 μU/mL	assay range accuracy: 86-87% (Mouse Serum), accuracy: 88-92% (Rat Serum), accuracy: 93-95% (Rat Plasma), linearity: 86-108%, standard curve range: 25-1600 pM	assay range accuracy: 104.2%, linearity: 100.8%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
detection method colorimetric (450nm/590nm)	detection method colorimetric (450nm)	detection method colorimetric (450nm/590nm)	detection method colorimetric (450nm/590nm)
technique(s) ELISA: suitable	technique(s) ELISA: suitable	technique(s) ELISA: suitable	technique(s) ELISA: suitable

General description

Insulin is secreted by the islet β cells in the pancreas. It contains two peptide chains linked by disulfide bridges. The A chain is composed of 21 amino acids and the B chain has 30 amino acids.

Application

Rat/Mouse Insulin ELISA is suitable for measuring and quantifying insulin levels in metabolism and endocrine research. It has been used to determine plasma insulin by enzyme-linked immunosorbent assay. It has also been used to measure the insulin secretion from cells, in vitro.

Biochem/physiol Actions

Insulin is a key hormone that maintains normal blood glucose levels. It mediates cellular glucose uptake, controls carbohydrate, lipid, and protein metabolism, and enhances cell division and growth through its mitogenic effects. Insulin resistance is a condition of impaired sensitivity to insulin-mediated glucose disposal.

Other Notes

3 hour assay, room temperature 10 μL sample size
Research Category: Metabolism
Research Sub Category: Obesity/Metabolic Disorders

Disclaimer

For research use only. Not for use in diagnostic procedures.

pictograms

GHS05,GHS07

signalword

Warning

hcodes

H290,H317

pcodes

P234 - P261 - P272 - P280 - P302 + P352 - P333 + P313

Hazard Classifications

Met. Corr. 1 - Skin Sens. 1

Storage Class

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chronic hypoxia increases insulin-stimulated glucose uptake in mouse soleus muscle.

Gamboa JL, Garcia-Cazarin ML, Andrade FH

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology null

Amadori-glycated phosphatidylethanolamine, a potential marker for hyperglycemia, in streptozotocin-induced diabetic rats.

Sookwong P, Nakagawa K, Fujita I, Shoji N, Miyazawa T.

Lipids null

Offspring metabolomic response to maternal protein restriction in a rat model of intrauterine growth restriction (IUGR).

Alexandre-Gouabau, Marie-Cecile, et al.

Journal of Proteome Research, 10, 3292-3302 (2011)

Hypoxia-induced intrauterine growth restriction increases the susceptibility of rats to high-fat diet-induced metabolic syndrome.

Rueda-Clausen CF, Dolinsky VW, Morton JS, Proctor SD, Dyck JR, Davidge ST.

Diabetes null

Differentiation of mouse nuclear transfer embryonic stem cells into functional pancreatic beta cells.

W Jiang, Z Bai, D Zhang, Y Shi, J Yong, S Chen, M Ding, H Deng

Diabetologia null

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