FCMAB186F

Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1

clone B-Ly1, Milli-Mark^®, from mouse

• Synonym(s): Membrane-spanning 4-domains subfamily A member 1 ; ; B-lymphocyte surface antigen B1 ; ; Leukocyte surface antigen Leu-16 ; ; Bp35 ; ; CD_antigen=CD20
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: B-Ly1, monoclonal
• species reactivity: human
• manufacturer/tradename: Milli-Mark^®
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_690605.1
• UniProt accession no.: P11836
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MS4A1(931)

Description

General description

33 kDa (Zhou, 1995)

CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells (Tedder, 1994). In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines (Zhou, 1995). The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface (Tedder, 1994). Antibodies reacting with CD20 cytoplasmic epitopes are designated CD20cy (Zhou, 1995). It is suggested that CD20 plays a direct role in regulating the transmembrane conductive Ca 2+ flux of B cells which indicates a possible function for CD20 as a regulator of proliferation and differentiation (Tedder, 1994).

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1 is validated for use in FC for the detection of CD20.

Biochem/physiol Actions

Antibody recognizes Human CD20 antigen.

Physical form

Protein A purified

Purified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Preparation Note

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Human Lymphocytes

Evaluated by flow cytometry using human lymphocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable