FCMAB197F
Milli-Mark® Anti-CD43-FITC Antibody, clone DF-T1
clone DF-T1, Milli-Mark®, from mouse
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Synonym(s):
Leukosialin, Leukocyte sialoglycoprotein, Sialophorin, Galactoglycoprotein, GALGP
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.44
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
DF-T1, monoclonal
species reactivity
human
manufacturer/tradename
Milli-Mark®
technique(s)
flow cytometry: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SPN(6693)
Related Categories
General description
CD43 is a human heavily 0-glycosylated transmembrane protein, which is expressed by T cells and myeloid lineage cells (Borche, 1987; Stross, 1989). Reactivity with plasma cells has been observed (Beschomer, 1999). The molecule is known to be defective in the Wiskott-Aldrich syndrome and may participate in T-cell activation (Stross, 1989).
Immunoblotting studies have revealed molecular mass heterogeneity displayed by the antigen extracted from white blood cells of different lineage. Thus, two major bands of 140 and 130 kDa are seen for the promyelocytic cell line HL60, 110 kDa for the myeloblast cell line KG1 and a major 115 kDa band for peripheral blood lymphocytes (Stross, 1989).
Immunoblotting studies have revealed molecular mass heterogeneity displayed by the antigen extracted from white blood cells of different lineage. Thus, two major bands of 140 and 130 kDa are seen for the promyelocytic cell line HL60, 110 kDa for the myeloblast cell line KG1 and a major 115 kDa band for peripheral blood lymphocytes (Stross, 1989).
Specificity
Antibody recognizes Human CD43 antigen.
Immunogen
Myeloblast cell line KG1
Application
Detect CD43 using this Milli-Mark Anti-CD43-FITC Antibody, clone DF-T1 validated for use in FC.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Quality
Evaluated by flow cytometry using human PBMCs
Target description
110 kDa (Stross, 1989)
Physical form
Protein A purified
Purified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Storage and Stability
Maintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
Analysis Note
Control
PBMCs
PBMCs
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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