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GF308

Sigma-Aldrich

NT-3 Protein, Human Recombinant Animal Free

Recombinant animal free human NT-3 is manufactured using all non-animal reagents.

Synonym(s):
NT3, Neurotrophin 3, HDNF, Nerve growth factor 2, Neurotrophic factor
eCl@ss:
32160405

Quality Level

assay

>98% (SDS-PAGE and HPLC)

impurities

<0.1 μg/mg protein endotoxin (<1EU/μg)

UniProt accession no.

shipped in

dry ice

General description

Neurotrophin3 (NT3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT3, and NT4/5. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6 kDa polypeptide monomers (240 total amino acid residues).Human and Mouse NT-3 sequences are identical.
Product Source: E. coli

Specificity

Cross Reactivty
None

Application

Recombinant animal free human NT-3 is manufactured using all non-animal reagents.
Research Category
Neuroscience
Research Sub Category
Growth Factors & Receptors

Quality

Bioactivity assay: The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found in the range of 20-50 ng/ml.

Physical form

Recombinant animal free human NT-3 is manufactured using all non-animal reagents
Product is filtered through a 0.2 micron filter before lyophilization. Product is presented in .

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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