GF310

PDGF-BB Protein, Human Recombinant Animal Free

The Platelet derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. Recombinant animal free human PDGF-BB is manufactured using all non-animal reagents.

• Synonym(s): Platelet Derived Growth Factor-BB, Platelet Derived Growth Factor, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.77

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• assay: >98% (SDS-PAGE and HPLC)
• impurities: <0.1 μg/mg protein endotoxin (<1EU/μg)
• UniProt accession no.: P01127
• shipped in: dry ice

Description

General description

Platelet derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the αgranules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGFAA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed α and β. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Product Source: E. coli

Application

Research Category
Inflammation & Immunology

Research Sub Category
Growth Factors & Receptors

Biochem/physiol Actions

Cross Reactivty
Cow, Monkey, Mouse, Pig, Rat

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human PDGF-BB is manufactured using all non-animal reagents

Preparation Note

Store at -20°C for up to 6 months from date of receipt.

Analysis Note

Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1