This product contains solids lyophilized from a solution with 10 mM sodium phosphate at pH 7.4 and 0.5 mM DTT.
GF314
TNF- α Protein, Human Recombinant Animal Free
Tumor necrosis factor alpha (TNF-α), also known as cachectin & TNFSF1A, is the prototypic ligand of the TNF superfamily. Recombinant animal free human TNF-α is manufactured using all non-animal reagents.
Synonym(s):
Tumor Necrosis Factor Alpha, TNFa, TNF-alpha, Cachectin, TNFSF1A
Sign In to View Organizational & Contract Pricing.
About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
eCl@ss:
32160405
Assay:
>98% (SDS-PAGE and HPLC)
Recombinant:
expressed in E. coli
Skip To
recombinant
expressed in E. coli
assay
>98% (SDS-PAGE and HPLC)
impurities
<0.1 μg/mg protein endotoxin (<1EU/μg)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
1 of 4
This Item | GF317 | 654245 | GF307 |
|---|---|---|---|
| assay >98% (SDS-PAGE and HPLC) | assay >98% (SDS-PAGE and HPLC) | assay ≥97% (SDS-PAGE) | assay >98% (SDS-PAGE and HPLC) |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - |
| impurities <0.1 μg/mg protein endotoxin (<1EU/μg) | impurities <0.1 μg/mg protein endotoxin (<1 EU/μg) | impurities ≤100 pg/μg Endotoxin (pg/μg TNF-α) | impurities <0.1 μg/mg protein endotoxin (<1EU/μg) |
| shipped in dry ice | shipped in dry ice | shipped in ambient | shipped in dry ice |
| UniProt accession no. | UniProt accession no. | UniProt accession no. - | UniProt accession no. |
| NCBI accession no. | NCBI accession no. - | NCBI accession no. - | NCBI accession no. |
General description
Product Source: E. coli
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. Recombinant human TNF-α is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
Cross Reactivty
Cow, Monkey, Mouse, Rat, Sheep
Cow, Monkey, Mouse, Rat, Sheep
Physical form
Product is filtered through a 0.2 micron filter before lyophilization.
Recombinant animal free human TNF- α is manufactured using all non-animal reagents
Preparation Note
Store at -20°C for up to 6 months from date of receipt.
Analysis Note
Bioactivity assay: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥2 x 107 units/mg.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Toll-like receptor 9 (TLR9) gene deletion-mediated fracture healing in type II diabetic osteoporosis associates with inhibition of the nuclear factor-kappa B (NF-I?B) signaling pathway.
Han, et al.
Bioengineered, 13, 13689-13702 (2022)
Bjarne Østerud et al.
Journal of thrombosis and haemostasis : JTH, 20(4), 866-876 (2021-11-26)
Most tissue factor (TF) activity assays are based on measurement of factor X (FX) activation by TF in the presence of factor VII (FVII)/FVIIa. This requires long incubation, which may result in TF-independent activity of FX and inaccurate measurement of
Ruo-Yu Chen et al.
Cell death & disease, 12(11), 983-983 (2021-10-24)
Chronic and persistent inflammation is a well-known carcinogenesis promoter. Hepatocellular carcinoma (HCC) is one of the most common inflammation-associated cancers; most HCCs arise in the setting of chronic inflammation and hepatic injury. Both NF-κB and STAT3 are important regulators of
Zhihua Ye et al.
Cell death & disease, 13(7), 633-633 (2022-07-22)
Uncontrolled growth, distant metastasis and chemoresistance are critical characteristics of pancreatic ductal adenocarcinoma (PDAC), and they result in high mortality; however, the mechanisms triggering these effects have not been fully investigated. In this study, we analysed a dataset in the
Ruo-Yu Chen et al.
Cell death and differentiation, 27(4), 1259-1273 (2019-09-13)
Centrosomal P4.1-associated protein (CPAP) is overexpressed in hepatocellular carcinoma (HCC) and positively correlated with recurrence and vascular invasion. Here, we found that CPAP plays an important role in HCC malignancies. Functional characterization indicated that CPAP overexpression increases tumor growth, angiogenesis
-
What additives (e.g. DTT, trehalose, Histidine, Tris etc.) are included in solution prior to filtering / lyophilization?
1 answer-
Helpful?
-
-
What is the recommended method for reconstituting item GF314, the Sigma-Aldrich TNF-α Protein, Human Recombinant Animal Free?
1 answer-
The lyophilized Tumor Necrosis Factor-alpha should be reconstituted in sterile 18MΩ-cm H2O to a concentration of not less than 100µg/ml. This solution can then be further diluted to other aqueous solutions.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service