HumanKine® Thermostable bFGF,
Human Recombinant

Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.

FGF2, prostatropin, Prostatropin, HBGF-2, BFGF

Quality Level


>95% (SDS-PAGE)




<0.5 μg/mg protein endotoxin (<5 EU/μg)

UniProt accession no.

General description

Product Source: HumanKine growth factors are expressed in a proprietary human 293 protein expression system and are manufactured without the use of any animal products and are considered Xeno-Free.
The HumanKine Thermostable bFGF is engineered with enchanced thermostability compared to the wild-type bFGF protein without modification of biological function.

Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 154 amino acid residues. bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions.


Research Category
Stem Cell Research
HumanKine products are Xeno-Free
Engineered bFGF growth factor with enchanced thermostability compared to the wild-type bFGF protein. Allows a less vigorous feeding schedule for human es/ips and neural stem cell culture.
Research Sub Category
Growth Factors & Receptors


Bioactivity assay: Determined by the dose-dependent stimulation of the proliferation of the 3T3 cell line. ED50 is typically <0.5 ng/mL.

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.

Storage and Stability

Store at -20°C for up to 4 months from date of receipt. Further dilutions should be made into appropriate buffer or medium to which protein has been added.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Fibroblast growth factors (FGFs) are secreted glycoproteins that regulate several fundamental developmental pathways and help regulate mesoderm and ectoderm patterning in the early embryonic development.
Read More
The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.
Read More
Products to enable the creation of neural models specific for your research.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.