HADCYMAG-61K
MILLIPLEX® Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay
The Human Adipocyte Magnetic Bead Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 11 Adipocyte protein biomarkers in cell culture samples.
About This Item
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Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 100%
(IL-6)
accuracy: 102%
(TNFα)
accuracy: 103%
(MCP-1)
accuracy: 83%
(PAI-1 (total))
accuracy: 99%
(HGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; IL-8)
sensitivity: 0.2 pg/mL
(MinDC+2SD; NGF)
sensitivity: 0.2 pg/mL
(MinDC+2SD; TNF?)
sensitivity: 0.6 pg/mL
(MinDC+2SD; IL-6)
sensitivity: 10 pg/mL
(MinDC+2SD; Leptin)
sensitivity: 15 pg/mL
(MinDC+2SD; Adiponectin)
sensitivity: 2.0 pg/mL
(MinDC+2SD; IL-1?)
sensitivity: 2.1 pg/mL
(MinDC+2SD; MCP-1)
sensitivity: 3.0 pg/mL
(MinDC+2SD; HGF)
sensitivity: 4.4 pg/mL
(MinDC+2SD; Resistin)
sensitivity: 4.6 pg/mL
(MinDC+2SD; PAI-1 (total))
standard curve range: 0.29-4,000 pg/mL
(IL-8)
standard curve range: 0.32-5,000 pg/mL
(NGF)
standard curve range: 0.38-6,000 pg/mL
(TNFα)
standard curve range: 0.51-8,000 pg/mL
(IL-1β)
standard curve range: 0.96-150,000 pg/mL
(IL-6)
standard curve range: 0.96-150,000 pg/mL
(MCP-1)
standard curve range: 26-400,000 pg/mL
(Adipnectin)
standard curve range: 6.4-100,000 pg/mL
(HGF)
standard curve range: 6.4-100,000 pg/mL
(Resistin)
standard curve range: 9.6-150,000 pg/mL
(Leptin)
standard curve range: 9.6-150,000 pg/mL
(PAI-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Adiponectin)
inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)
inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)
inter-assay cv: <15%
intra-assay cv: <10%
(Resistin)
inter-assay cv: <20%
intra-assay cv: <10%
(HGF)
inter-assay cv: <20%
intra-assay cv: <10%
(IL-6)
inter-assay cv: <20%
intra-assay cv: <10%
(NGF)
inter-assay cv: <20%
intra-assay cv: <10%
(PAI-1 (total))
inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)
inter-assay cv: <20%
intra-assay cv: <15%
(IL-1β)
inter-assay cv: <25%
intra-assay cv: <20%
(Leptin)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
The MILLIPLEX® Human Adipocyte Panel is an 11-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cell/tissue culture samples: Adiponectin, HGF, IL-β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (total), Resistin, TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Specificity
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Application
- Analytes: Adiponectin, HGF, IL-1β, IL-6, IL-8, Leptin, MCP-1, NGF, PAI-1 (Total), Resistin, TNF-α
- Recommended Sample Type: Tissue/cell culture supernatants or extracts
- Recommended Sample Dilution: 25 μL per well of undiluted tissue/cell culture sample; samples may require dilution with an appropriate medium prior to assay
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Endocrine
Features and Benefits
Packaging
Storage and Stability
Other Notes
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Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificates of Analysis (COA)
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