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HAGP1MAG-12K

Millipore

MILLIPLEX® Human Angiogenesis/Growth Factor Magnetic Bead Panel - Cancer Multiplex Assay

Angiogenesie Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple angiogenic biomarkers in human serum, plasma and cell culture samples.

eCl@ss:
32161000


To order a Milliplex kit, please search for your analyte of interest.


Design And Price Your Kit

Quality Level

species reactivity

human

mfr. no.

MILLIPLEX®

assay range

accuracy: 42-131%
standard curve range: 1.4-1,000 pg/mL
(HB-EGF, IL-8, PLGF)

standard curve range: 13.7-10,000 pg/mL
(Angiopoietin-2, FGF-1, FGF-2, G-CSF, VEGF-A)

standard curve range: 137.2-100,000 pg/mL
(Leptin)

standard curve range: 2.7-2,000 pg/mL
(BMP-9, EGF, Endothelin-1)

standard curve range: 27.4-20,000 pg/mL
(Endoglin, Follistatin, HGF)

standard curve range: 6.9-5,000 pg/mL
(VEGF-C, VEGF-D)

application(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Angiogenesis, the development of new vascular networks, is a key process in normal growth and development as well as in wound healing, with homeostasis maintained by a delicate balance of angiogenic factors and inhibitors. Consequently, insufficient, or excessive blood vessel growth underlies many diseases, including cardiovascular disease, diabetic ulcers, macular degeneration, and cancer. Angiogenesis plays a significant role in tumor growth and metastasis.

The MILLIPLEX® Human Angiogenesis / Growth Factor Panel 1 is to be used for the simultaneous quantification of any or all of the following 17 human angiogenesis and growth factor biomarkers in human plasma, serum samples and tissue/cell lysate and culture supernatant samples: Angiopoietin-2, BMP-9, EGF, Endoglin, Endothelin-1, FGF-1 (acidic FGF), FGF-2 (basic FGF), Follistatin, G-CSF, HB-EGF, HGF, IL-8, Leptin, PLGF, VEGF-A, VEGF-C and VEGF-D. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer

Specificity

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: Angiopoietin-2, BMP-9, EGF, Endoglin, Endothelin-1, FGF-1 (acidic FGF), FGF-2 (basic FGF), Follistatin, G-CSF, HB-EGF, HGF, IL-8, Leptin, PLGF, VEGF-A, VEGF-C, VEGF-D
  • Recommended Sample Type: Human serum, plasma, tissue/cell lysate or culture supernatants
  • Recommended Sample Dilution: 25 μL per well of a 1:3 dilution of plasma or serum; 25 μL cell culture supernatant per well, diluted with appropriate control medium as needed
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual analytes.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sonali Chaudhury et al.
Frontiers in pediatrics, 7, 475-475 (2019-12-05)
Objective: To test the hypothesis that umbilical cord blood-derived CD34+ hematopoietic stem cells (HPSC), cord tissue-derived CD90+ and CD105+ mesenchymal stem cells (MSC) vary with bronchopulmonary dysplasia (BPD). Methods: We conducted a prospective longitudinal study at a large birth center...

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