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HATF00010

Immobilon® -NC Nitrocellulose Membrane

1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, nitrocellulose-based transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, nitrocellulose transfer membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
mixed cellulose esters (MCE) membrane, nitrocellulose membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

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Product Name

Immobilon®-NC Transfer Membrane, 1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

Quality Level

400

material

mixed cellulose esters (MCE) membrane, nitrocellulose membrane, plain filter, white filter

feature

hydrophilic

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

33 cm × 3 m

pore size

0.45 μm pore size

capacity

117 μg/cm2 adsorption capacity (insulin), 160 μg/cm2 adsorption capacity (BSA), 259 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

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HATF00010

Immobilon®-NC Transfer Membrane

MCE Membrane Filter, 0.45 μm Pore Size Immobilon®, filter diam. 142 mm, hydrophilic

Millipore

HATF14250

MCE Membrane Filter, 0.45 μm Pore Size

Immobilon®-NC Transfer Membrane 47 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane

Millipore

HATF04700

Immobilon®-NC Transfer Membrane

MCE Membrane Filter, 0.45 μm Pore Size Immobilon®, filter diam. 13 mm, hydrophilic

Millipore

HATF01300

MCE Membrane Filter, 0.45 μm Pore Size

material

mixed cellulose esters (MCE) membrane, plain filter, nitrocellulose membrane, white filter

material

mixed cellulose esters (MCE) membrane (surfactant-free), plain filter, white filter

material

mixed cellulose esters (MCE) membrane (surfactant-free), nitrocellulose membrane, plain filter, white filter

material

mixed cellulose esters (MCE) membrane (surfactant-free), plain filter, white filter

pore size

0.45 μm pore size

pore size

0.45 μm pore size, 79 % porosity

pore size

0.45 μm pore size, 79 % porosity

pore size

0.45 μm pore size, 79 % porosity

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

compatibility

for use with Amido black, for use with Colloidal gold, for use with Ponceau-S red, for use with CPTS, for use with India ink

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with India ink, for use with Ponceau-S red

detection method

chemiluminescent, fluorometric, radioactive, colorimetric

detection method

colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

colorimetric, fluorometric, radioactive

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®, Millipore

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®, Millipore

General description

Immobilon-NC (HATF) membrane is composed of a mixed cellulose esters matrix. It is devoid of surfactants that may interfere with cell wall integrity during cell growth. This membrane is recommended for colony and plaque lifts.

Application

Immobilon-NC Transfer Membrane has been used in western blot analysis.[1][2][3][4]

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Danger

hcodes

H228

Hazard Classifications

Flam. Sol. 1

Storage Class

4.1B - Flammable solid hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yunhua Hou et al.
Experimental cell research, 387(2), 111750-111750 (2019-12-02)
Lymphoma, a malignant tumor, is mainly characterized by painless lymph node enlargement and hepatosplenomegaly. At present, lymphoma is mainly treated by radiation, chemical drugs, bone marrow transplantation and surgery. However, due to the high degree of heterogeneity, lymphomas are highly
1H-Indole-3-Carbonyl-Thiazole-4-Carboxylic Acid Methyl Ester Blocked Human Glioma Cell Invasion via Aryl Hydrocarbon Receptor?s Regulation of Cytoskeletal Contraction
Zhao L, et al.
BioMed Research International (2020)
Kanika Verma et al.
Nature communications, 11(1), 2926-2926 (2020-06-12)
Metabolic changes alter the cellular milieu; can this also change intracellular protein folding? Since proteostasis can modulate mutational buffering, if change in metabolism has the ability to change protein folding, arguably, it should also alter mutational buffering. Here we find
Bing Wang et al.
Oncology letters, 13(5), 3494-3500 (2017-05-23)
The aim of the present study was to gain insight into the molecular mechanism of gefitinib resistance in non-small cell lung cancer (NSCLC), and demonstrate whether long noncoding RNA (lncRNA) expression signatures differ between gefitinib-sensitive PC9 and gefitinib-resistant PC9 (PC9-R)
Jianxin Liu et al.
The Journal of international medical research, 48(6), 300060520925598-300060520925598 (2020-06-13)
To investigate the anti-metastatic effects of Babao Dan (BBD) on gastric cancer (GC) cells (AGS and MGC80-3) and explore the underlying molecular mechanisms by which it inhibits epithelial-mesenchymal transition (EMT). AGS and MGC80-3 cells were treated with BBD. In addition
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Protocols

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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