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HCCBP1MAG-58K

Millipore

MILLIPLEX® Human Circulating Cancer Biomarker Magnetic Bead Panel - Cancer Multiplex Assay

Circulating Cancer Biomarker Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple oncology biomarkers in various tumor types in human serum, plasma and cell culture samples.

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Synonym(s):
human cancer biomarker immunoassay panel, luminex human circulating cancer biomarkers multiplex assay, millipore human circulating cancer biomarkers multiplex kit
eCl@ss:
32161000

Quality Level

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 35-108%
standard curve range: 0.09-67 mU/mL
(β-HCG)

standard curve range: 0.7-500 pg/mL
(IL-6)

standard curve range: 1.3-1000 pg/mL
(IL-8, TNFα)

standard curve range: 2.7-2000 pg/mL
(TRAIL, TGF-α)

standard curve range: 6.9-5000 pg/mL
(SCF)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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HCCBP3MAG-58KHCCBP2MAG-58KHAGP1MAG-12K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 35-108%, standard curve range: 0.09-67 mU/mL
(β-HCG), standard curve range: 0.7-500 pg/mL
(IL-6), standard curve range: 1.3-1000 pg/mL
(IL-8, TNFα), standard curve range: 2.7-2000 pg/mL
(TRAIL, TGF-α), standard curve range: 6.9-5000 pg/mL
(SCF)

assay range

accuracy: 74-126%, standard curve range: 0.004-3 ng/mL
(Galectin-3), standard curve range: 0.02-20 ng/mL
(Myeloperoxidase), standard curve range: 0.03-25 nM
(Sex Hormone Binding Globulin), standard curve range: 0.03-25 ng/mL
(Ferritin), standard curve range: 0.07-50 ng/mL
(Catalase, Fibroblast Activation Protein α), standard curve range: 0.1-75 ng/mL
(Melanoma Inhibitory Activity Protein), standard curve range: 0.27-200 ng/mL
(Cathepsin D, IGFBP3)

assay range

accuracy: 92-107%, standard curve range: 0.01-50 ng/mL
(Extracellular matrix protein 1), standard curve range: 0.48-2,000 ng/mL
(All other biomarkers)

assay range

accuracy: 42-131%, standard curve range: 1.4-1,000 pg/mL
(HB-EGF, IL-8, PLGF), standard curve range: 13.7-10,000 pg/mL
(Angiopoietin-2, FGF-1, FGF-2, G-CSF, VEGF-A), standard curve range: 137.2-100,000 pg/mL
(Leptin), standard curve range: 2.7-2,000 pg/mL
(BMP-9, EGF, Endothelin-1), standard curve range: 27.4-20,000 pg/mL
(Endoglin, Follistatin, HGF), standard curve range: 6.9-5,000 pg/mL
(VEGF-C, VEGF-D)

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

MILLIPLEX® Circulating Cancer Biomarker Panel 1 is a 25-plex kit that can be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell lysate and culture supernatant samples: AFP, CA125, CA15-3, CA19-9, CEA, CYFRA21-1, sFas, sFasL, FGF2, β-HCG, HE4, HGF, IL-6, IL-8, Leptin, MIF, Osteopontin, Prolactin, PSA (free), PSA (total), SCF, TGFα, TNFα, TRAIL and VEGF. Note that certain biomarkers are tumor specific, such as PSA, while others, such as IL-8, have been detected in many cancers. PSA (free) and PSA (total) cannot be plexed together. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer

Specificity

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: α-Fetoprotein (AFP), CA125, CA15-3, CA19-9, CEA, CYFRA21-1, sFas (TNFRSF6), sFasL, FGF-2 (FGF-basic), β-HCG (HCGβ), HE4, HGF, IL-6, IL-8 (CXCL8), Leptin, MIF, Osteopontin (OPN), Prolactin, PSA (free)*, PSA (total)*, SCF, TGFα, TNFα, TRAIL (TNFSF10), VEGF.
  • Note: Total PSA and Free PSA cannot be plexed together
  • Recommended Sample Type: Human serum, plasma or tissue/cell culture supernatants or lysates
  • Recommended Sample Dilution: 25 μL per well of 1:6 diluted plasma or serum, or 25 μL cell culture psaesaa sample undiluted or diluted in appropriate control medium
  • Assay Run Time: Overnight (16-18 hr) at 2-8°C
  • Research Category: Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Components

Human Circulating Cancer Biomarker Panel 1 Standard (HCC-8058)

Human Circulating Cancer Biomarker Panel 1 Quality Controls 1 and 2 (HCC-6058)

Serum Matrix (HCP-SM)
Note: Contains 0.08% Sodium Azide

Set of one 96-Well Plate with 2 Sealers (MAG-PLATE)

Assay Buffer (L-AB)
Note: Contains 0.08% Sodium Azide

10X Wash Buffer (L-WB)
Note: Contains 0.05% Proclin

Human Circulating Cancer Biomarker Panel 1 Detection Antibodies (HCC-1058 or HCC-1058-2)

Streptavidin-Phycoerythrin (L-SAPE 4)

Bead Diluent (LBD-4)

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please note: Total PSA and Free PSA cannot be plexed together.
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Chen-Tzu Chiu et al.
Cancers, 12(6) (2020-06-18)
Head and neck cancer (HNC) accounts for more than 330,000 cancer deaths annually worldwide. Despite late diagnosis being a major factor contributing to HNC mortality, no satisfactory biomarkers exist for early disease detection. Cytoplasmic gelsolin (cGSN) was discovered to predict
A combined blood based gene expression and plasma protein abundance signature for diagnosis of epithelial ovarian cancer--a study of the OVCAD consortium.
Pils, D; Tong, D; Hager, G; Obermayr, E; Aust, S; Heinze, G; Kohl, M; Schuster, E; Wolf et al.
BMC Cancer null
Effect of different exercise modalities plus a hypocaloric diet on inflammation markers in overweight patients: a randomised trial.
Loria-Kohen, V; Fernandez-Fernandez, C; Bermejo, LM; Morencos, E; Romero-Moraleda et al.
Clinical Nutrition (Edinburgh, Scotland) null
Daniel Ajona et al.
Translational research : the journal of laboratory and clinical medicine, 233, 77-91 (2021-02-23)
Lung cancer screening detects early-stage cancers, but also a large number of benign nodules. Molecular markers can help in the lung cancer screening process by refining inclusion criteria or guiding the management of indeterminate pulmonary nodules. In this study, we
A J Prati et al.
Journal of dental research, 92(12 Suppl), 161S-167S (2013-10-26)
The aim of this study was to compare the release of bone markers during osseointegration of immediately loaded and nonloaded implants. Forty patients who were indicated for rehabilitation with dental implants randomly received either implant and prosthesis placement within 72

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