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MILLIPLEX® Human Complement Panel 1 - Immunology Multiplex Assay

The Human Complement Panel 1 Bead-Based Multiplex Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of complement proteins and factors in human serum, plasma and cell culture samples.

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Human complement multiplex kit, Luminex® human complement immunoassay, Millipore human complement panel

Quality Level

species reactivity




assay range

accuracy: 96%
(Mannose-Binding Lectin (MBL))

sensitivity: 0.0051 ng/mL
(Complement C5a)

sensitivity: 0.026 ng/mL
(Adipsin (Complement Factor D))

sensitivity: 0.040 ng/mL
(Mannose-Binding Lectin (MBL))

sensitivity: 0.22 ng/mL
(Complement Factor I)

sensitivity: 0.31 ng/mL
(Complement C2)

sensitivity: 0.36 ng/mL
(Complement C4b)

sensitivity: 1.040 ng/mL
(Complement C5)

standard curve range: 0.069-50 ng/mL
(Adipson/ Complement Factor D)

standard curve range: 0.07-50 ng/mL
(Complement C2)

standard curve range: 0.137-100 ng/mL
(Mannose-Binding Lectin (MBL))

standard curve range: 0.69-500 ng/mL
(Complement Factor I)

standard curve range: 1.37-1,000 ng/mL
(Complement C4b)

standard curve range: 2.74-2,000 ng/mL
(Complement C5)

standard curve range: 4.12-30,000 pg/mL
(Complement C5a)


multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in


General description

The complement system consists of many plasma proteins that assist the ability of phagocytic cells and antibodies to clear pathogens. There are three different simultaneous pathways. The classical pathway is stimulated by antigen-antibody complexes; the alternative pathway spontaneously activates on contact with pathogenic cell surfaces; and the mannose-binding lectin (MBL) pathway recognizes mannose sugars usually present only on pathogenic cell surfaces. Because of the potential to be extremely damaging to host tissues, complement system activation must be tightly controlled. The regulating complement control proteins, including CD59, also known as protectin, are present at a higher concentration in the blood plasma than the complement proteins themselves. The complement system is thought to play a key role in many diseases with an immune component, such as asthma and many autoimmune diseases, including SLE, IBD and multiple sclerosis. It is also becoming increasingly associated with neurological disease, such as Alzheimer′s disease and conditions such as spinal cord injuries.

MILLIPLEX® Complement Panel 1 Bead Panel is an 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma or culture supernatant samples: C2, C4B, C5, C5a, Adipsin/Complement Factor D, MBL and Complement Factor I.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response


Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.


  • Analytes: C2, C4b, C5, C5a, Adipsin/Factor D, Mannose-Binding Lectin (MBL), and Factor I
  • Recommended Sample type: Serum, plasma, and tissue/cell culture supernatant
  • Recommended Sample dilution: 1:200 diluted plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
  • Assay Run Time: Overnight or two-hour primary incubation
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Inflammation
  • Note: To avoid complement activation in samples, which will affect the results, samples should be thawed as rapidly as possible, put on ice and assayed immediately

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.


96 well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.



Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2


Respiratory Tract

Storage Class

10 - Combustible liquids



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