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HCVD3MAG-67K

Millipore

MILLIPLEX® Human Cardiovascular Disease (Acute Phase) Magnetic Bead Panel 3 - Cardiovascular Disease Multiplex Assay

The analytes available for this multiplex kit are: Adipsin, AGP, α2-Macroglobulin, CRP, Fetuin A, Fibrinogen, L-Selectin, Serum Amyloid P, Haptoglobin, & Platelet Factor-4.

eCl@ss:
32161000


To order a Milliplex kit, please search for your analyte of interest.


Design And Price Your Kit

Quality Level

species reactivity

human

mfr. no.

MILLIPLEX®

assay range

accuracy: 102%
(Haptoglobin)

accuracy: 109%
(AGP)

accuracy: 113%
(Platelet Factor-4)

accuracy: 117%
(Adipsin)

standard curve range: 0.004-15 ng/mL
(L-Selectin & SAP)

standard curve range: 0.012-50 ng/mL
(CRP)

standard curve range: 0.037-150 ng/mL
(PF4)

standard curve range: 0.061-250 ng/mL
(HPTGN)

standard curve range: 0.098-400 ng/mL
(AGP & Adipsin)

standard curve range: 0.244-1,000 ng/mL
(Fetuin A & vWF)

standard curve range: 0.488-2,000 ng/mL
(A2M)

application(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Cardiovascular disease, particularly atherosclerotic vascular disease, is a leading cause of global mortality, accounting for 30% of all global deaths (WHO, 2010). Inflammatory mechanisms play a vital role in the initiation, maintenance, and progression of vascular disease with a strong correlation between inflammatory markers and prognosis in acute and chronic coronary artery disease. Numerous studies have demonstrated an association of obesity and diabetes with cardiovascular risk factors.

MILLIPLEX® Human Cardiovascular Disease (CVD) Panel 3 (Acute Phase) is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma or tissue/cell lysate and culture supernatant samples: Adipsin, α-1-Acid-Glycoprotein (AGP), α-2-Macroglobulin (A2M), C Reactive Protein (CRP), Fetuin A, Fibrinogen, L-Selectin, Serum Amyloid P (SAP), Haptoglobin, and Platelet Factor-4 (PF4). (Please note: Fibrinogen is not detectable in serum.) This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cardiovascular

Application

  • Analytes: α-1 Acid Glycoprotein (AGP), α-2-Macroglobulin (A2M), Adipsin (Factor D), C Reactive Protein (CRP), Fetuin A, Fibrinogen, Haptoglobin, sL-Selectin, Platelet Factor-4 (PF4/CXCL4), Serum Amyloid P (SAP)
  • Recommended Sample Type: Human serum, plasma, cell/tissue culture supernatants and lysates
  • Recommended Sample Dilution: 25 μL per well of 1:40,000 diluted serum or plasma; tissue/cell culture samples may require dilution in appropriate control medium
  • NOTE: Fibrinogen is not detectable in serum
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25 °C)
  • Research Category: Cardiovascular Disease
  • Research Subcategory: Metabolic Disorders

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Sensitivity: See kit protocol for individual analyte sensitivity.
Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Coagulation factors, fibrinogen and plasminogen activator inhibitor-1, are differentially regulated by yellow fever virus infection of hepatocytes.
Woodson, SE; Freiberg, AN; Holbrook, MR
Virus Research null
Kévin Contrepois et al.
Cell, 181(5), 1112-1130 (2020-05-30)
Acute physical activity leads to several changes in metabolic, cardiovascular, and immune pathways. Although studies have examined selected changes in these pathways, the system-wide molecular response to an acute bout of exercise has not been fully characterized. We performed longitudinal...
Julia E Herrmann et al.
Toxicology and applied pharmacology, 274(2), 302-312 (2013-11-30)
Human response to isoproterenol induced cardiac injury was evaluated by gene and protein pathway changes in human heart slices, and compared to rat heart slices and rat heart in vivo. Isoproterenol (10 and 100μM) altered human and rat heart slice...

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