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HCYTOMAG-60K

Millipore

MILLIPLEX® Human Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

eCl@ss:
32161000


To order a Milliplex kit, please search for your analyte of interest.


Design And Price Your Kit

Quality Level

species reactivity

human

manufacturer/tradename

MILLIPLEX®

assay range

accuracy: 87-107%
standard curve range: 3.2-10,000 pg/mL
inter-assay cv: 5-19%
intra-assay cv: 2-13%

application(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.

The MILLIPLEX® Human Cytokine / Chemokine Panel 1 enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: sCD40L, EGF, Eotaxin/CCL11, FGF-2, Flt-3 ligand, Fractalkine, G-CSF, GM-CSF, GRO, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MCP-3, MDC (CCL22), MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TGF-α, TNF-α, TNF-β, VEGF
  • Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant samples
  • Recommended Sample dilution: Neat plasma or serum. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay. RANTES, PDGF-AA and PDGF-AB/BB require a 1:100 dilution of plasma/serum samples.
  • NOTE: RANTES, PDGF-AA and PDGF-AB/BB cannot be plexed with other cytokines in this panel due to a required 1:100 dilution of plasma/serum samples.
  • Assay Run Time: Overnight or two-hour primary incubation. For best results, an overnight incubation is recommended
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Obesity, Metabolic Disorders, Inflammation & Autoimmune Mechanisms

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96 well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities for individual cytokines/chemokines.
Please note: Sample Type must be selected when configuring this kit. RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mark E Scott et al.
International journal of cancer, 133(5), 1187-1196 (2013-02-26)
Mechanisms for the control and resolution of human papillomavirus (HPV) infection of the cervix include the local production of cytokines, which control recruitment and function of cells integral to pathogen control. We established a cohort of women for long-term follow-up...
Paolo Cassano et al.
The Australian and New Zealand journal of psychiatry, 51(1), 23-31 (2016-06-18)
There is mixed evidence in the literature on the role of inflammation in major depressive disorder. Contradictory findings are attributed to lack of rigorous characterization of study subjects, to the presence of concomitant medical illnesses, to the small sample sizes...
K L Jones et al.
Molecular psychiatry, 22(2), 273-279 (2016-05-25)
Immune abnormalities have been described in some individuals with autism spectrum disorders (ASDs) as well as their family members. However, few studies have directly investigated the role of prenatal cytokine and chemokine profiles on neurodevelopmental outcomes in humans. In the...
Intra- and inter-day variation of cytokines and chemokines in tears of healthy subjects.
Benito, MJ; Gonzalez-Garcia, MJ; Teson, M; Garcia, N; Fernandez, I; Calonge et al.
Experimental Eye Research null
Maternal obesity is associated with a lipotoxic placental environment.
Saben, J; Lindsey, F; Zhong, Y; Thakali, K; Badger, TM; Andres, A; Gomez-Acevedo, H; Shankar, K
Placenta null

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