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HNABTMAG-68K

Millipore

MILLIPLEX® Human Amyloid Beta and Tau Magnetic Bead Panel - Multiplex Assay

The MILLIPLEX® MAP Human Amyloid Beta and Tau Panel is a 4-plex kit containing all reagents needed for simultaneous quantification of Aβ40, Aβ42, Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181) in CSF samples.

eCl@ss:
32161000


To order a Milliplex kit, please search for your analyte of interest.


Design And Price Your Kit

Quality Level

species reactivity

human

mfr. no.

MILLIPLEX®

assay range

accuracy: 109%
(Spike recovery for Aβ1-42)

accuracy: 91%
(Spike recovery for pTau181)

accuracy: 98%
(Spike recovery for tTau)

sensitivity: 1.5 pg/mL
(pTau181; MinDC+2SD)

sensitivity: 10.2 pg/mL
(A?1-40; MinDC+2SD)

sensitivity: 14.2 pg/mL
(tTau; MinDC+2SD)

sensitivity: 2.5 pg/mL
(A?1-42; MinDC+2SD)

standard curve range: 0.7-500 pg/mL
(pTau181)

standard curve range: 11-8000 pg/mL
(tTau)

standard curve range: 2-2000 pg/mL
(Aβ1-42)

standard curve range: 21-15000 pg/mL
(Aβ1-40)

inter-assay cv: <15%
intra-assay cv: <10%

application(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

MILLIPLEX® Human Amyloid Beta and Tau Panel enables you to focus on the relevance and therapeutic potential of Aβ40, Aβ42, Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181) in your research model. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.

The MILLIPLEX® Human Amyloid Beta Tau Bead Panel is to be used for the simultaneous quantification of Aβ40, Aβ42, tTau, and pTau181. This kit may be used only for the analysis of cerebrospinal fluid (CSF). This is a configurable assay, and some or all analytes can be selected: tau (total), amyloid β40, amyloid β42 and tau (pThr181).

MILLIPLEX® portfolio offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

MILLIPLEX® Human Amyloid Beta Tau Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate, and build the most comprehensive library available for protein detection and quantitation.

Panel Type: Neuroscience

Specificity

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Application

  • Analytes: Tau (Total), Amyloid β40, Amyloid β42, Tau (pThr181)
  • Recommended Sample type: cerebrospinal fluid (CSF)
  • Recommended Sample dilution: 1:2 sample dilution is recommended for extracted CSF samples
  • Assay Run Time: Overnight
  • Research Category: Neuroscience Metabolism
  • Research Subcategory: Metabolic Disorders Obesity Inflammation & Autoimmune Mechanisms
  • Research Category Neuroscience Metabolism
  • NOTE: This is a competitive assay

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.
96-well plate

Components

Human ABeta and Tau Standard, 1 vial, lyophilized

Human ABeta and Tau Quality Control 1, 1 vial, lyophilized

Human ABeta and Tau Quality Control 2, 1 vial, lyophilized

Bead Diluent, 1 bottle, 3.5mL

Set of one 96-Well Plate with 2 sealers

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Human ABeta and Tau Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Protocols
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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