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HNABTMAG-68K

Millipore

MILLIPLEX® Human Amyloid Beta and Tau Magnetic Bead Panel - Multiplex Assay

allows quantitative multiplex detection of multiple analytes simultaneously

Synonym(s):

Human Neuroscience Protein Multiplex Assay, Luminex® Human Neuroscience Panel, Millipore Human Neuroscience Immunoassay

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
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Quality Level

200

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 109%
(Spike recovery for Aβ1-42)
accuracy: 91%
(Spike recovery for pTau181)
accuracy: 98%
(Spike recovery for tTau)
sensitivity: 1.5 pg/mL
(pTau181; MinDC+2SD)
sensitivity: 10.2 pg/mL
(Aβ1-40; MinDC+2SD)
sensitivity: 14.2 pg/mL
(tTau; MinDC+2SD)
sensitivity: 2.5 pg/mL
(Aβ1-42; MinDC+2SD)
standard curve range: 0.7-500 pg/mL
(pTau181)
standard curve range: 11-8000 pg/mL
(tTau)
standard curve range: 2-2000 pg/mL
(Aβ1-42)
standard curve range: 21-15000 pg/mL
(Aβ1-40)
inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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MILLIPLEX&#174; Human Amyloid Beta and Tau Magnetic Bead Panel - Multiplex Assay allows quantitative multiplex detection of multiple analytes simultaneously

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species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

assay range

accuracy: 109%
(Spike recovery for Aβ1-42), accuracy: 98%
(Spike recovery for tTau), sensitivity: 10.2 pg/mL
(A?1-40; MinDC+2SD), sensitivity: 2.5 pg/mL
(A?1-42; MinDC+2SD), standard curve range: 11-8000 pg/mL
(tTau), standard curve range: 21-15000 pg/mL
(Aβ1-40), inter-assay cv: <15%
intra-assay cv: <10%, sensitivity: 1.5 pg/mL
(pTau181; MinDC+2SD), standard curve range: 0.7-500 pg/mL
(pTau181), accuracy: 91%
(Spike recovery for pTau181), standard curve range: 2-2000 pg/mL
(Aβ1-42), sensitivity: 14.2 pg/mL
(tTau; MinDC+2SD)

assay range

accuracy: 98-118%
(Spike Recovery), sensitivity: 11-479 pg/mL
(MinDC+2SD), standard curve range: 27-20,000 pg/mL
(α-MSH), standard curve range: 41-30,000 pg/mL
(Neurotensin), standard curve range: 55-40,000 pg/mL
(Oxytocin), standard curve range: 69-50,000 pg/mL
(β-Endorphine), standard curve range: 7-5,000 pg/mL
(Substance P)

assay range

accuracy: 108%
(RANTES), accuracy: 108%
(sICAM-1), accuracy: 111%
(Cathepsin D), accuracy: 123%
(PDGF-AA), accuracy: 137%
(PDGF-AB/BB), standard curve range: 2-10,000 pg/mL
(RANTES), standard curve range: 2.4-10,000 pg/mL
(BDNF), standard curve range: 2.4-10,000 pg/mL
(PAI-1 (total)), standard curve range: 2.4-10,000 pg/mL
(PDGF-AA), standard curve range: 24-100,000 pg/mL
(Cathepsin D), standard curve range: 24-100,000 pg/mL
(MPO), standard curve range: 24-100,000 pg/mL
(NCAM), standard curve range: 24-100,000 pg/mL
(PDGF-AB/BB), standard curve range: 24-100,000 pg/mL
(sICAM-1), standard curve range: 61-250,000 pg/mL
(sVCAM-1)

assay range

accuracy: 85%
(Apo E), accuracy: 86%
(Apo AI), accuracy: 89%
(Apo CIII), accuracy: 92%
(Apo B), sensitivity: 1.00 ng/mL
(Apo AII), sensitivity: 1.22 ng/mL
(Apo CII), sensitivity: 1.42 ng/mL
(Apo E), sensitivity: 1.48 ng/mL
(Apo AI), sensitivity: 1.71 ng/mL
(Apo CIII), sensitivity: 4.84 ng/mL
(Apo B)

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

MILLIPLEX® Human Amyloid Beta and Tau Panel enables you to focus on the relevance and therapeutic potential of Aβ40, Aβ42, Total Tau proteins (tTau), and Phosphorylated Tau Thr181 (pTau181) in your research model. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.

The MILLIPLEX® Human Amyloid Beta Tau Bead Panel is to be used for the simultaneous quantification of Aβ40, Aβ42, tTau, and pTau181. This kit may be used only for the analysis of cerebrospinal fluid (CSF). This is a configurable assay, and some or all analytes can be selected: tau (total), amyloid β40, amyloid β42 and tau (pThr181).

MILLIPLEX® portfolio offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) provided to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

MILLIPLEX® Human Amyloid Beta Tau Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate, and build the most comprehensive library available for protein detection and quantitation.

Panel Type: Neuroscience

Application

  • Analytes: Tau (Total), Amyloid β40, Amyloid β42, Tau (pThr181)
  • Recommended Sample type: cerebrospinal fluid (CSF)
  • Recommended Sample dilution: 1:2 sample dilution is recommended for extracted CSF samples
  • Assay Run Time: Overnight
  • Research Category: Neuroscience Metabolism
  • Research Subcategory: Metabolic Disorders Obesity Inflammation & Autoimmune Mechanisms
  • Research Category Neuroscience Metabolism
  • NOTE: This is a competitive assay

Biochem/physiol Actions

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96-well plate
Everything you need in a single kit.

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Other Notes

Human ABeta and Tau Standard, 1 vial, lyophilized

Human ABeta and Tau Quality Control 1, 1 vial, lyophilized

Human ABeta and Tau Quality Control 2, 1 vial, lyophilized

Bead Diluent, 1 bottle, 3.5mL

Set of one 96-Well Plate with 2 sealers

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Human ABeta and Tau Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mohamed Raâfet Ben Khedher et al.
Alzheimer's & dementia (New York, N. Y.), 7(1), e12124-e12124 (2021-02-05)
In brain, extracellular vesicles (EVs) play an essential role in the neuron-glia interface and ensure the crosstalk between the brain and the periphery. Some studies now link the pathway dysfunction of the EVs to apolipoprotein E gene variant (APOE ε4)
Jessica L Hoffman et al.
International review of neurobiology, 148, 169-230 (2019-11-18)
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that represents the most common cause of dementia in the United States. Although the link between alcohol use and AD has been studied, preclinical research has potential to elucidate neurobiological mechanisms that
Francheska Delgado-Peraza et al.
Cells, 10(5) (2021-05-01)
Circulating neuronal extracellular vesicles (NEVs) of Alzheimer's disease (AD) patients show high Tau and β-amyloid (Aβ) levels, whereas their astrocytic EVs (AEVs) contain high complement levels. To validate EV proteins as AD biomarkers, we immunocaptured NEVs and AEVs from plasma
Wencheng Yin et al.
Frontiers in neuroscience, 14, 602642-602642 (2021-01-05)
Alzheimer's disease (AD)-related degenerative decline is associated to the presence of amyloid beta (Aβ) plaque lesions and neuro fibrillary tangles (NFT). However, the precise molecular mechanisms linking Aβ deposition and neurological decline are still unclear. Here we combine genome-wide transcriptional
Barbara Miziak et al.
Pharmaceuticals (Basel, Switzerland), 14(5) (2021-06-03)
Alzheimer's disease (AD; progressive neurodegenerative disorder) is associated with cognitive and functional impairment with accompanying neuropsychiatric symptoms. The available pharmacological treatment is of a symptomatic nature and, as such, it does not modify the cause of AD. The currently used
View All Related Papers

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Questions

1–7 of 7 Questions  

  1. Can the Human Amyoid Beta / Tau (HNABTMAG-68K) Milliplex kit, which currently only lists CSF as a sample type, be used with serum/plasma samples?

    1 answer

    1. The HNABTMAG-68K Milliplex kit currently only lists cerebrospinal fluid (CSF) as a sample type. When serum/plasma samples were tested with this kit, a strong matrix effect was observed. As the kit was not validated for serum/plasma, the appropriate Serum Matrix for use could not be ascertained. Furthermore, the expected trend for Amyloid β40 and Amyloid β42 in serum/plasma was not observed.

      For serum/plasma samples, we recommend using the validated ELISA kits for Amyloid β40 and 42 (EZHS and EZH42) as they are the preferred assays. Additionally, we offer a Total Tau ELISA (RAB1085).

      Helpful?

  2. Could you provide the plate capacity for an LX200 or FM3D instrument when using one box of Sheath Fluid?

    1 answer

    1. The FLEXMAP 3D and LX200 instruments use approximately 10 mL of sheath per sample run when running a 96-well plate and the xPONENT protocol is set to aspirate 50 µL per sample. Therefore, to acquire one 96-well plate, the instruments will use approximately 1L of sheath fluid.

      Helpful?

  3. Is it possible to add other analytes to the Human Amyloid Beta / Tau Panel (HNABTMAG-68K) as SPR kits?

    1 answer

    1. Additional analytes cannot be added to the AB/Tau panel due to differences in the assay procedure. The detection antibody is added at the primary incubation, whereas the other panels have the detection added after the primary incubation.

      Helpful?

  4. What is the recommended concentration of guanidine for Amyloid Beta Immunoassays?

    1 answer

    1. The recommended guanidine concentration for Amyloid Beta Immunoassays should be less than 0.5M in the final sample concentration.

      Helpful?

  5. Are the antibodies used in the Human Amyloid Beta/Tau (HNABTMAG-68K) kit also able to recognize rat samples?

    1 answer

    1. The AB40 and AB42 antibodies have not been specifically tested for reactivity to rat. However, in a test involving 4 rat serum samples, AB40 was detected in all of the samples, while AB42 was not detectable. Additionally, the capture antibodies for pTau and Total Tau have been found to be reactive to both human and rat, as pTau and Total Tau were detected in 2 out of the 4 rat serum samples tested. It's important to note that the panel has not been tested with rat standards to determine the extent of cross-reactivity.

      Helpful?

  6. Can you provide information on the specificity or cross reactivity of Total Tau in the human HNABTMAG-68K?

    1 answer

    1. The human panel HNABTMAG-68k follows the Tau standard with isoform Tau441. Both the capture and detection immunogens used are also Tau441. Cross reactivity for other isoforms has not been tested.

      Helpful?

  7. How should the plate for the complete Milliplex assay in Sheath Fluid be stored if it can't be read for a couple of hours?

    1 answer

    1. The plate can be stored at 4°C without shaking for up to 24 hours. Before reading, the plate should be shaken at room temperature for at least 20 minutes to bring it back to ambient temperature.

      Helpful?

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