The kits cannot be combined after purchase because the detection antibodies are provided at a 1X concentration. Combining reagents would dilute out the detection antibody cocktails, impacting the signal and sensitivity. Additionally, there is a risk of cross-reactivity, and the matrix conditions may vary depending on the kit. We do offer an SPR program for combining panels and can review requests prior to the purchase of the kits.
HNPMAG-35K
MILLIPLEX® Human Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay
The analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.
Synonym(s):
Human Neuroscience Protein Multiplex Assay, Luminex® Human Neuroscience Panel, Millipore Human Neuroscience Immunoassay
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
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Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 98-118%
(Spike Recovery)
sensitivity: 11-479 pg/mL
(MinDC+2SD)
standard curve range: 27-20,000 pg/mL
(α-MSH)
standard curve range: 41-30,000 pg/mL
(Neurotensin)
standard curve range: 55-40,000 pg/mL
(Oxytocin)
standard curve range: 69-50,000 pg/mL
(β-Endorphine)
standard curve range: 7-5,000 pg/mL
(Substance P)
technique(s)
multiplexing: suitable
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
1 of 4
This Item | HNDG3MAG-36K | HNABTMAG-68K | APOMAG-62K |
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| assay range accuracy: 98-118% | assay range accuracy: 108% | assay range accuracy: 109% | assay range accuracy: 85% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) | detection method fluorometric (Luminex xMAP) |
General description
The central nervous system is a complex environment consisting of billions of neurons and glial support cells. These neurons use chemical signals to communicate information. Neuropeptides are neuronal signaling molecules secreted by the central and peripheral nervous systems, influencing a broad spectrum of biological functions including the regulation of metabolism, reproduction, immunity, and behavior. Understanding the role of neuropeptides in the pathogenesis of devastating neuronal diseases and behavioral disorders is fundamental. While it is understood that accurate measurement of neuropeptides is important, conventional methods, including RIAs and ELISAs, are not able to simultaneously measure multiple neuropeptides with small sample volume. MILLIPLEX® Human Neuropeptide Bead Panel is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cerebrospinal fluid (CSF), tissue/cell lysate, and culture supernatant samples: α-Melanocyte Stimulating Hormone (α-MSH), β-Endorphin, Neurotensin, Oxytocin, and Substance P.
The MILLIPLEX® Human Neuropeptide Bead Panel is to be used for the simultaneous quantification of the following 5 analytes in any combination: α-MSH, β-Endorphin, Neurotensin, Oxytocin, and Substance P. This kit may be used for the analysis of all or any combination of the above analytes in tissue/cell lysate, culture supernatant samples, and cerebrospinal fluid (CSF). Serum or plasma samples may also be used and will need to be extracted. An important note about this conversion panel is that the samples may be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with the kit.
Panel Type: Neuroscience
The MILLIPLEX® Human Neuropeptide Bead Panel is to be used for the simultaneous quantification of the following 5 analytes in any combination: α-MSH, β-Endorphin, Neurotensin, Oxytocin, and Substance P. This kit may be used for the analysis of all or any combination of the above analytes in tissue/cell lysate, culture supernatant samples, and cerebrospinal fluid (CSF). Serum or plasma samples may also be used and will need to be extracted. An important note about this conversion panel is that the samples may be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with the kit.
Panel Type: Neuroscience
Application
- Analytes: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P
- Note that this is a competitive assay.
- Recommended Sample type: serum, plasma, tissue culture supernatant, or CSF
- Recommended Sample dilution: extracted serum or plasma or neat CSF or culture supernatant
- Assay Run Time: Overnight
- Research Category: Neuroscience
Biochem/physiol Actions
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Features and Benefits
Design your multiplex kit by choosing available analytes within this panel.
Packaging
Everything you need in a single kit.
Preparation Note
Recommended storage for kit components is 2 - 8°C.
Other Notes
Human Neuropeptide Standard, 1 vial, lyophilized
Human Neuropeptide Quality Control 1, 1 vial, lyophilized
Human Neuropeptide Quality Control 2, 1 vial, lyophilized
Assay Buffer, 2 bottles, 30 mL ea.
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Human Neuropeptide Primary Antibodies, 1 bottle, 3.5 mL
Human Neuropeptide Detection Antibodies, 1 bottle, 5.5 mL
Human Neuropeptide Matrix Solution, 1 bottle, 1.5 mL
Human Neuropeptide Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
Human Neuropeptide Quality Control 1, 1 vial, lyophilized
Human Neuropeptide Quality Control 2, 1 vial, lyophilized
Assay Buffer, 2 bottles, 30 mL ea.
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Human Neuropeptide Primary Antibodies, 1 bottle, 3.5 mL
Human Neuropeptide Detection Antibodies, 1 bottle, 5.5 mL
Human Neuropeptide Matrix Solution, 1 bottle, 1.5 mL
Human Neuropeptide Antibody-Immobilized Magnetic Beads, 20X, 200 uL ea
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate with 2 Foil Plate Sealers
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Immediate effects of spinal manipulation on nitric oxide, substance P and pain perception.
Molina-Ortega, F; Lomas-Vega, R; Hita-Contreras, F; Plaza Manzano, G; Achalandabaso et al.
Manual therapy null
Marco Barbariga et al.
Investigative ophthalmology & visual science, 59(3), 1305-1312 (2018-04-07)
The purpose of this study was to investigate the role of substance P (SP) in patients affected with corneal neovascularization (CNV) and in three different Tac1-knockout (KO) murine models of CNV. SP levels in tears were measured with a multiplex
Ann M Manzardo et al.
American journal of medical genetics. Part A, 170(9), 2328-2333 (2016-08-16)
Prader-Willi syndrome (PWS) is a rare genetic neurodevelopmental disorder associated with maladaptive social behavior, hyperphagia and morbid obesity. Orexin A is a hypothalamic neuropeptide important as a homeostatic regulator of feeding behavior and in energy metabolism through actions in the
Emmanuel Bäckryd et al.
Pain medicine (Malden, Mass.), 15(1), 111-119 (2013-10-15)
Pain medicine still lacks mechanism-specific biomarkers to guide diagnosis and treatment, and defective top-down modulation is an important factor in the pathophysiology of chronic pain conditions. Using modern analytical tools and advanced multivariate statistical analysis, the aim of this study
Olga V Roschina et al.
Journal of clinical medicine, 10(23) (2021-12-11)
The neuropeptides β-endorphin and oxytocin are released into the bloodstream as hormones from the pituitary gland but also have an important function as neuroregulators in the forebrain. The blood levels of both polypeptides have been shown to reflect depressive symptoms.
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Can reagents from two or more Milliplex kits be combined and run in a single plate if the bead regions do not overlap and if the samples use the same dilution factor?
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