The kit has been verified for use with CSF and cell/tissue culture supernatants. Protein normalization is not required for these sample types. If using the kit with cell or tissue lysates, the sample will need to be normalized to total protein, using either a BCA or Coomassie-based assay. The product has not been validated for use with cell/tissue lysates.
HNS1MAG-95K
MILLIPLEX® Human Neuroscience Panel
Configurable Human Neuroscience 6-Plex Panel 1
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.47
eCl@ss:
32161000
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Product Name
MILLIPLEX® Human Neuroscience Panel,
species reactivity
human
Quality Level
packaging
pkg of 1 ea
manufacturer/tradename
Milliplex®
assay range
accuracy: 107%
(DJ1), accuracy: 108%
(a-Synuclein), accuracy: 123%
(TG2), standard curve range: 12-50,000 pg/mL
(GFAP), standard curve range: 15-60,000 pg/mL
(NSE), standard curve range: 20-80,000 pg/mL
(UCHL1), standard curve range: 244-1,000,000 pg/mL
(alpha-synuclein), standard curve range: 61-250,000 pg/mL
(DJ1), standard curve range: 61-250,000 pg/mL
(TG2)
technique(s)
multiplexing: suitable
input
cell culture supernatant
cerebrospinal fluid(s) (CSF)
detection method
fluorometric (Luminex® xMAP® technology)
shipped in
wet ice
storage temp.
2-8°C
description
Configurable Human Neuroscience 6-Plex Panel 1
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This Item | |||
|---|---|---|---|
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| assay range accuracy: 107% | assay range accuracy: 84-108%, standard curve range: 14-10,000 pg/mL | assay range accuracy: 69-92% | assay range accuracy: 124% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level - |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| shipped in ambient | shipped in wet ice | shipped in wet ice | shipped in - |
General description
Cellular stress and structural damage in the brain are reflected in specific protein signatures. GFAP signals astrocyte activation and blood-brain barrier disruption. UCHL1, a neuron-specific enzyme, increases after traumatic brain injury. α-Synuclein, central to Parkinson′s pathology, affects synaptic function and aggregates into Lewy bodies. DJ1 (PARK7) mitigates oxidative stress, while NSE and TGM2 track neuronal integrity and cellular resilience.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 6 analytes in human cerebrospinal fluid (CSF).
Analytes included: α-Synuclein, DJ1/PARK7, Glial fibrillary acidic protein (GFAP), Neuron specific enolase (NSE), Transglutaminase 2 (TGM2), Ubiquitin carboxyl-terminal esterase L1 (UCHL1/PARK5).
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Analytes included: α-Synuclein, DJ1/PARK7, Glial fibrillary acidic protein (GFAP), Neuron specific enolase (NSE), Transglutaminase 2 (TGM2), Ubiquitin carboxyl-terminal esterase L1 (UCHL1/PARK5).
Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Features and Benefits
- Comprehensive Neurological Assessment: Simultaneously quantify six critical biomarkers spanning neuronal damage, glial activation, and protein aggregation pathways, providing complete neurological dysfunction profiling in cerebrospinal fluid samples.
- Parkinson′s Disease Research Focus: Leverage specialized biomarkers including α-synuclein and DJ1/PARK7 that are directly implicated in Parkinson′s pathology, enabling targeted research into movement disorders and synucleinopathies.
- CSF-Optimized Performance: Specifically designed and verified for cerebrospinal fluid analysis, ensuring accurate quantification of brain-derived biomarkers in this challenging but clinically relevant sample matrix.
- Cross-Condition Biomarker Power: Utilize markers relevant to traumatic brain injury, neurodegenerative diseases, and neuroinflammation, maximizing research versatility across diverse neurological conditions and study designs.
- Configurable Panel Flexibility: Design your ideal research solution by selecting any combination of the 6 analytes, creating tailored assays that perfectly align with your specific neurological research objectives.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
For research use only. Not for use in diagnostic procedures.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Label License/Sticker for Assay Product:
By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
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Daniela Diaz-Lucena et al.
Journal of neurology, 267(9), 2567-2581 (2020-05-07)
Differential diagnosis of neurodegenerative dementia is currently supported by biomarkers including cerebrospinal fluid (CSF) tests. Among them, CSF total-tau (t-tau), phosphorylated tau (p-tau) and β-amyloid42 (Aβ42) are considered core biomarkers of neurodegeneration. In the present work, we hypothesize that simultaneous
Martin Trapecar et al.
Science advances, 7(5) (2021-01-31)
Slow progress in the fight against neurodegenerative diseases (NDs) motivates an urgent need for highly controlled in vitro systems to investigate organ-organ- and organ-immune-specific interactions relevant for disease pathophysiology. Of particular interest is the gut/microbiome-liver-brain axis for parsing out how
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How can I normalize protein for this test? Just using same volume and total concentration protein are not reliable.
1 answer-
Helpful?
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Can serum or plasma samples be utilized in the Human Neuroscience Panel 1 kit, considering that the protocol exclusively mentions CSF?
1 answer-
The kit has not been validated with serum/plasma samples. Eight normal plasma samples were tested at a 1:4 dilution. It was found that GFAP and UCHL1 will require more sensitivity.
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