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HSP1MAG-63K

Millipore

MILLIPLEX® Human Sepsis Magnetic Bead Panel 1 - Immune Response Multiplex Assay

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.

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eCl@ss:
32161000

Quality Level

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 111%
(sVCAM-1)

accuracy: 114%
(MIF)

accuracy: 98%
(sFas)

standard curve range: 12-50,000 pg/mL
(sFas, sFasL, and PAI-1)

standard curve range: 61-250,000 pg/mL
(sVCAM-1 and sICAM-1)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

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HSP2MAG-63KHCVD2MAG-67KHCVD4MAG-67K
manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 111%
(sVCAM-1), accuracy: 114%
(MIF), accuracy: 98%
(sFas), standard curve range: 12-50,000 pg/mL
(sFas, sFasL, and PAI-1), standard curve range: 61-250,000 pg/mL
(sVCAM-1 and sICAM-1)

assay range

accuracy: 87%
(MIP-1β), accuracy: 92%
(MIP-1α), accuracy: 95%
(Granzyme B), accuracy: 98%
(IL-8), standard curve range: 10-10,000 pg/mL
(IL-1α and MIP-1β), standard curve range: 195-200,000 pg/mL
(MMP-8), standard curve range: 2,930-3,000,000 pg/mL
(HSP70), standard curve range: 2-2,000 pg/mL
(Granzyme B), standard curve range: 5-5,000 pg/mL
(IL-8 and MIP-1β)

assay range

accuracy: 88%
(Myoglobin), accuracy: 90%
(P-selectin), accuracy: 98%
(GDF-15), standard curve range: 0.0005-2.5 ng/mL
(GDF-15), standard curve range: 0.012-50 ng/mL
(Lipocalcin-2/NGAL), standard curve range: 0.024-100 ng/mL
(Myoglobin & MPO), standard curve range: 0.085-350 ng/mL
(sICAM-1), standard curve range: 0.122-500 ng/mL
(sVCAM-1), standard curve range: 0.244-1,000 ng/mL
(D-dimer, SAA & P-selectin), standard curve range: 1.221-5,000 ng/mL
(ADAMTS 13), inter-assay cv: <20%
intra-assay cv: <15%

assay range

accuracy: 103%
(Thrombomodulin), accuracy: 104%
(Pentraxin-3 (PTX3)), accuracy: 104%
(Tissue Factor (TF)), accuracy: 106%
(Pecam-1), accuracy: 109%
(sE-Selectin), standard curve range: 0.01-5 ng/mL
(Troponin T (TnT)), standard curve range: 0.04-30 ng/mL
(Follistatin (FST)), standard curve range: 0.14-100 ng/mL
(Pentraxin-3 (PTX3) & Tissue Factor (TF)), standard curve range: 0.27-200 ng/mL
(Pecam-1), standard curve range: 0.34-250 ng/mL
(Thrombomodulin), standard curve range: 2.7-2,000 ng/mL
(sE-Selectin), inter-assay cv: <20%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Sepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal, or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. The pathogenesis of sepsis is very complex. Both an innate and acquired immune response to infection interrupts homeostasis with a shift of cytokine expression that increases immunosuppression. In addition, migration of the leukocytes in the inflamed tissues secretes a tissue factor leading to the formation of thrombin, also triggering the release of chemokines and adhesion molecules through endothelial cells. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death.

MILLIPLEX® Human Sepsis Panel 1 is to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma and cell / tissue culture supernatant samples: sFAS/TNFRSF6, sFasL, sICAM-1, MIF, PAI-1 (total), sVCAM-1. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Application

  • Analytes: MIF, PAI-1 (Total), sFas, sFasL, sICAM-1, sVCAM-1
  • Recommended Sample type: Serum, plasma, tissue culture, or homogenates
  • Recommended Sample dilution: 1:10 for serum/plasma
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Sensitivity: Refer to kit protocol for individual sensitivities.
Standard Curve Range: Refer to QC analysis sheet for exact concentration (MIF).

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Pictograms

Exclamation markEnvironment

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Sigita Kazune et al.
Critical care (London, England), 23(1), 311-311 (2019-09-13)
In patients with septic shock, the skin is often chosen for the evaluation of peripheral perfusion and oxygenation. Changes in skin microcirculatory vessel oxygen saturation and relative hemoglobin concentration can be described using a mottling score or captured with hyperspectral
Dai Kimura et al.
Frontiers in pediatrics, 4, 31-31 (2016-04-12)
Lung injury activates multiple pro-inflammatory pathways, including neutrophils, epithelial, and endothelial injury, and coagulation factors leading to acute respiratory distress syndrome (ARDS). Low-dose methylprednisolone therapy (MPT) improved oxygenation and ventilation in early pediatric ARDS without altering duration of mechanical ventilation
Miša Korva et al.
Viruses, 11(8) (2019-07-31)
Hemorrhagic fever with renal syndrome (HFRS) and Crimean-Congo hemorrhagic fever (CCHF) are important viral hemorrhagic fevers (VHF), especially in the Balkan region. Infections with Dobrava or Puumala orthohantavirus and Crimean-Congo hemorrhagic fever orthonairovirus can vary from a mild, nonspecific febrile
Sonia Y Velásquez et al.
The Journal of biological chemistry, 291(25), 12960-12977 (2016-04-30)
Natural killer (NK) cells induce apoptosis in infected and transformed cells and are important producers of immunoregulatory cytokines. Therefore, they operate under low oxygen conditions (hypoxia) in inflammatory and tumor environments. In vitro studies of NK cells are, however, commonly

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