MilliporeSigma
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HSTC384-28K

Millipore

MILLIPLEX® 384-well Human High Sensitivity T Cell Panel - Immunology Multiplex Assay

This kit may be used for the analysis of all or any combination of the 384-well Human High Sensitivity T Cell panel analytes in serum, plasma, or tissue/cell culture supernatant. Because there are several distinctive steps, please read the entire protocol before running the assay.

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32161000

Quality Level

species reactivity

human

manufacturer/tradename

Milliplex®

assay range

accuracy: 79-108%
standard curve range: 0.18-750 pg/mL
(IL-6)

standard curve range: 0.24-1,000 pg/mL
(IL-13)

standard curve range: 0.24-1,000 pg/mL
(IL-21)

standard curve range: 0.31-1,250 pg/mL
(IL-8)

standard curve range: 0.31-1,250 pg/mL
(MIP-1α)

standard curve range: 0.37-1,500 pg/mL
(IL-7)

standard curve range: 0.43-1,750 pg/mL
(TNFα)

standard curve range: 0.49-2,000 pg/mL
(IL-1β)

standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70))

standard curve range: 0.49-2,000 pg/mL
(IL-2)

standard curve range: 0.49-2,000 pg/mL
(IL-5)

standard curve range: 0.61-2,500 pg/mL
(IFNγ)

standard curve range: 0.61-2,500 pg/mL
(MIP-3α)

standard curve range: 0.73-3,000 pg/mL
(IL-17A)

standard curve range: 0.92-3,750 pg/mL
(MIP-1β)

standard curve range: 1.22-5,000 pg/mL
(GM-CSF)

standard curve range: 1.46-6,000 pg/mL
(IL-10)

standard curve range: 1.46-6,000 pg/mL
(ITAC)

standard curve range: 1.83-7,500 pg/mL
(IL-4)

standard curve range: 18.3-75,000 pg/mL
(Fractalkine)

standard curve range: 7.93-32,500 pg/mL
(IL-23)

inter-assay cv: <20%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

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manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

assay range

accuracy: 79-108%, standard curve range: 0.18-750 pg/mL
(IL-6), standard curve range: 0.24-1,000 pg/mL
(IL-13), standard curve range: 0.24-1,000 pg/mL
(IL-21), standard curve range: 0.31-1,250 pg/mL
(IL-8), standard curve range: 0.31-1,250 pg/mL
(MIP-1α), standard curve range: 0.37-1,500 pg/mL
(IL-7), standard curve range: 0.43-1,750 pg/mL
(TNFα), standard curve range: 0.49-2,000 pg/mL
(IL-1β), standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70)), standard curve range: 0.49-2,000 pg/mL
(IL-2), standard curve range: 0.49-2,000 pg/mL
(IL-5), standard curve range: 0.61-2,500 pg/mL
(IFNγ), standard curve range: 0.61-2,500 pg/mL
(MIP-3α), standard curve range: 0.73-3,000 pg/mL
(IL-17A), standard curve range: 0.92-3,750 pg/mL
(MIP-1β), standard curve range: 1.22-5,000 pg/mL
(GM-CSF), standard curve range: 1.46-6,000 pg/mL
(IL-10), standard curve range: 1.46-6,000 pg/mL
(ITAC), standard curve range: 1.83-7,500 pg/mL
(IL-4), standard curve range: 18.3-75,000 pg/mL
(Fractalkine), standard curve range: 7.93-32,500 pg/mL
(IL-23), inter-assay cv: <20%
intra-assay cv: <10%

assay range

accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.17-13,000 pg/mL
(LIX), standard curve range: 3.42-14,000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), inter-assay cv: <15%
intra-assay cv: <10%

assay range

accuracy: 98-107%, sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC), standard curve range: 0.18-750 pg/mL
(IL-6), standard curve range: 0.24-1,000 pg/mL
(IL-13), standard curve range: 0.24-1,000 pg/mL
(IL-21), standard curve range: 0.31-1,250 pg/mL
(IL-8), standard curve range: 0.31-1,250 pg/mL
(MIP-1α), standard curve range: 0.37-1,500 pg/mL
(IL-7), standard curve range: 0.45-1,750 pg/mL
(TNFα), standard curve range: 0.49-2,000 pg/mL
(IL-1β), standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70)), standard curve range: 0.49-2,000 pg/mL
(IL-2), standard curve range: 0.49-2,000 pg/mL
(IL-5), standard curve range: 0.61-2,500 pg/mL
(IFNγ), standard curve range: 0.61-2,500 pg/mL
(MIP-3α), standard curve range: 0.73-3,000 pg/mL
(IL-17A), standard curve range: 0.92-3,750 pg/mL
(MIP-1β), standard curve range: 1.22-5,000 pg/mL
(GM-CSF), standard curve range: 1.46-6,000 pg/mL
(IL-10), standard curve range: 1.46-6,000 pg/mL
(ITAC), standard curve range: 1.83-7,500 pg/mL
(IL-4), standard curve range: 18.3-75,000 pg/mL
(Fractalkine), standard curve range: 7.93-32,500 pg/mL
(IL-23), inter-assay cv: <15%
intra-assay cv: <10%

assay range

accuracy: 82-102%, sensitivity: 0.26-11.14 pg/mL, standard curve range: 0.10-400 pg/mL
(IL-4), standard curve range: 0.49-2,000 pg/mL
(IFNγ), standard curve range: 0.49-2,000 pg/mL
(IL-17A), standard curve range: 0.49-2,000 pg/mL
(IL-7), standard curve range: 0.49-2,000 pg/mL
(TNFα), standard curve range: 0.73-3,000 pg/mL
(KC/GROα/CXCL1), standard curve range: 0.98-4,000 pg/mL
(IL-2), standard curve range: 0.98-4,000 pg/mL
(IL-5), standard curve range: 0.98-4,000 pg/mL
(IL-6), standard curve range: 1.34-5,500 pg/mL
(IL-10), standard curve range: 2.93-12,000 pg/mL
(IL-12 (p70)), standard curve range: 3.17-13,000 pg/mL
(LIX), standard curve range: 3.42-14.000 pg/mL
(IL-13), standard curve range: 3.91-16,000 pg/mL
(IL-1α), standard curve range: 3.91-16,000 pg/mL
(IL-1β), standard curve range: 3.91-16,000 pg/mL
(MCP-1/CCL2), standard curve range: 6.1-25,000 pg/mL
(GM-CSF), standard curve range: 7.32-30,000 pg/mL
(MIP-2/CXCL2), inter-assay cv: <15%
intra-assay cv: <10%

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

shipped in

ambient

shipped in

wet ice

shipped in

ambient

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases. The 384-well Human High Sensitivity T Cell Panel is the 384-well version of the current 96-well Human High Sensitivity T Cell Panel, and it exceeds expectations in terms of throughput, sensitivity and sample detectability.

The MILLIPLEX® 384-well Human High Sensitivity T Cell Panel is to be used for the simultaneous quantification of any or all of the above analytes in serum, plasma, or tissue/cell culture supernatant: Fractalkine/CX3CL1, GM-CSF, IFN-gamma, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL8/CXCL8, IL-10, IL-12 (p70), IL-13, IL17A/CTLA8, IL-21, IL-23, ITAC/EXCL11, MIP-1α/CCL3, MIP-1β/CCL4, MIP-3α/CCL20 and TNFα.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
There is no detectable cross-reactivity within the panel.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, I-TAC, MIP-1α, MIP-1β, MIP-3α, TNF-α
  • Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
  • Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology
  • Research Subcategory: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease, Neuroscience

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Related Content

Multiplex Assays for COVID-19 (SARS-CoV-2) Research Applications | MILLIPLEX® Assays

See how MILLIPLEX® multiplex immunoassays offer researchers the ability to simultaneously quantitate a large number of human cytokines, chemokines, and growth factors, and detect human immunoglobulins against SARS-CoV-2 antigens, to better understand the immune response to COVID-19, including cytokine storm.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service