Product HX0514 is from bovine testes.
HX0514
Hyaluronidase
Synonym(s):
Hyaluronidase
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conjugate
conjugate (Glucosaminoglycan)
Quality Level
form
powder
specific activity
≥300 units/mg dry wt.
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
shipped in
ambient
storage temp.
−20°C
Related Categories
1 of 4
This Item | H2126 | H6254 | 389561 |
|---|---|---|---|
| specific activity ≥300 units/mg dry wt. | specific activity ≥300 units/mg solid | specific activity ≥1,500 units/mg solid | specific activity ≥2000 units (A280) |
| form powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage condition OK to freeze | storage condition - | storage condition - | storage condition OK to freeze |
| shipped in ambient | shipped in - | shipped in - | shipped in ambient |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
General description
300 units/mg minimum, 50,000 units/vial. Suitable for biochemistry grade.
Specifications:Activity: 300NF U/mg min
Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
Specifications:Activity: 300NF U/mg min
Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
Application
Hyaluronidase has been used:
- to enzymatically remove extra-cellular matrix (ECM) to assess the lateral diffusion of the synaptic a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR)[1]
- to study the effects of 4-methylumbelliferone (4MU)/ hyaluronidase (HLX)-mediated depletion of the CD44/hyaluronan complex on trastuzumab binding in JIMT1 breast cancer xenografts[2]
- as a component of Hank′s balanced salt solution (HBSS) medium to digest fetal intestine tissue[3]
Biochem/physiol Actions
Hyaluronidase is an endoglycosidase that degrades hyaluronic acid (HA), a component of the extracellular matrix. It is used to enhance drug diffusion and reverse the effects of HA filler injections. Hyaluronidases dissolve the subcutaneous nodules and can correct excessive quantities of injected filler. It eliminates the cumulus-corona-oocyte complex formed during intracytoplasmic sperm injection. Hyaluronidase inhibits tissue damage after the extravasation of several substances. It is used for reducing edema treating vitreous hemorrhage.[4]
Unit Definition
One unit is based on the change in absorbency (turbidity) at 540 nm of an internal standard assayed concurrently with each lot. Internal standard replaces USP/NF reference no longer available.
Physical form
Dialyzed, lyophilized powder, not less than300 units per mg (dry weight).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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what is its source?
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What is the appropriate way to reconstitute this? In what medium and at what concentration? Is there an information sheet that provides this information?
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The enzyme can be reconstituted at 1mg/ml in 0.1M Sodium phosphate buffer, pH 5.3 with 0.15M sodium chloride. Stock solutions can be aliquoted and frozen for several months at -20C.
Stock solutions prepared in water (30units/ml) are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
It is recommended to make hyaluronidase solutions fresh. The lyophilized powder is very stable, at least 2 years when stored frozen -20C. This product does not contain a product information sheet.
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