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HX0514

Sigma-Aldrich

Hyaluronidase

Synonym(s):

Hyaluronidase

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.78

$262.00


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conjugate

conjugate (Glucosaminoglycan)

Quality Level

form

powder

specific activity

≥300 units/mg dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

shipped in

ambient

storage temp.

−20°C

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This Item
H2126H6254389561
specific activity

≥300 units/mg dry wt.

specific activity

≥300 units/mg solid

specific activity

≥1,500 units/mg solid

specific activity

≥2000 units (A280)

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

shipped in

ambient

shipped in

-

shipped in

-

shipped in

ambient

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

OK to freeze

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

Calbiochem®

General description

300 units/mg minimum, 50,000 units/vial. Suitable for biochemistry grade.

Specifications:Activity: 300NF U/mg min





Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Application

Hyaluronidase has been used:
  • to enzymatically remove extra-cellular matrix (ECM) to assess the lateral diffusion of the synaptic a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor (AMPAR)[1]
  • to study the effects of 4-methylumbelliferone (4MU)/ hyaluronidase (HLX)-mediated depletion of the CD44/hyaluronan complex on trastuzumab binding in JIMT1 breast cancer xenografts[2]
  • as a component of Hank′s balanced salt solution (HBSS) medium to digest fetal intestine tissue[3]

Biochem/physiol Actions

Hyaluronidase is an endoglycosidase that degrades hyaluronic acid (HA), a component of the extracellular matrix. It is used to enhance drug diffusion and reverse the effects of HA filler injections. Hyaluronidases dissolve the subcutaneous nodules and can correct excessive quantities of injected filler. It eliminates the cumulus-corona-oocyte complex formed during intracytoplasmic sperm injection. Hyaluronidase inhibits tissue damage after the extravasation of several substances. It is used for reducing edema treating vitreous hemorrhage.[4]

Unit Definition

One unit is based on the change in absorbency (turbidity) at 540 nm of an internal standard assayed concurrently with each lot. Internal standard replaces USP/NF reference no longer available.

Physical form

Dialyzed, lyophilized powder, not less than300 units per mg (dry weight).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–2 of 2 Questions  
  1. what is its source?

    1 answer
    1. Product HX0514 is from bovine testes.

      Helpful?

  2. What is the appropriate way to reconstitute this? In what medium and at what concentration? Is there an information sheet that provides this information?

    1 answer
    1. The enzyme can be reconstituted at 1mg/ml in 0.1M Sodium phosphate buffer, pH 5.3 with 0.15M sodium chloride. Stock solutions can be aliquoted and frozen for several months at -20C.

      Stock solutions prepared in water (30units/ml) are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.

      It is recommended to make hyaluronidase solutions fresh. The lyophilized powder is very stable, at least 2 years when stored frozen -20C. This product does not contain a product information sheet.

      Helpful?

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